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Detection of vinblastine and related bioactive compounds from culture extracts of endophytic fungi of Catharanthus roseus
This study investigates the synthesis of vinblastine by endophytic fungi isolated from leaf of C. roseus . A total of 10 endophytic fungi were selected for secretion of vinca alkaloids based on the initial screening by biochemical tests and thin-layer chromatography (TLC). Out of these ten, only fou...
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| Published in: | World journal of microbiology & biotechnology 2024-09, Vol.40 (9), p.278, Article 278 |
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| creator | Khan, Uzma Gond, Surendra Kumar |
| description | This study investigates the synthesis of vinblastine by endophytic fungi isolated from leaf of
C. roseus
. A total of 10 endophytic fungi were selected for secretion of vinca alkaloids based on the initial screening by biochemical tests and thin-layer chromatography (TLC). Out of these ten, only four fungal extracts showed positive results for presence of vinblastine at same retention time (10 min.) compared to reference compound on HPLC analysis. The detected concentration of vinblastine was maximum (17 µg/ml) in isolate no. CRL 22 followed by CRL 52, CRL 17 and CRL 28. To validate the presence of vinblastine, ultra-high-performance liquid chromatography coupled with high-resolution accurate mass spectrometry (HRMS) was employed. This analysis confirmed the presence of anhydrovinblastine, a precursor of vinblastine through the detection of molecular ions at m/z 793.4185 in extract of CRL 17. In addition to anhydrovinblastine, the intermediate compounds essential to the biosynthetic pathway of vinblastine were also detected in the extract of CRL 17. These host-origin compounds strongly suggest the presence of a biosynthetic pathway within the endophytic fungus. Based on morphological observation and sequence analysis of the ITS region of rDNA, endophytic fungi were identified as
Alternaria alternata
(CRL 17),
Curvularia lunata
(CRL 28),
Aspergillus terrus
(CRL 52), and
Aspergillus clavatonanicus
(CRL 22). |
| doi_str_mv | 10.1007/s11274-024-04078-9 |
| format | article |
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C. roseus
. A total of 10 endophytic fungi were selected for secretion of vinca alkaloids based on the initial screening by biochemical tests and thin-layer chromatography (TLC). Out of these ten, only four fungal extracts showed positive results for presence of vinblastine at same retention time (10 min.) compared to reference compound on HPLC analysis. The detected concentration of vinblastine was maximum (17 µg/ml) in isolate no. CRL 22 followed by CRL 52, CRL 17 and CRL 28. To validate the presence of vinblastine, ultra-high-performance liquid chromatography coupled with high-resolution accurate mass spectrometry (HRMS) was employed. This analysis confirmed the presence of anhydrovinblastine, a precursor of vinblastine through the detection of molecular ions at m/z 793.4185 in extract of CRL 17. In addition to anhydrovinblastine, the intermediate compounds essential to the biosynthetic pathway of vinblastine were also detected in the extract of CRL 17. These host-origin compounds strongly suggest the presence of a biosynthetic pathway within the endophytic fungus. Based on morphological observation and sequence analysis of the ITS region of rDNA, endophytic fungi were identified as
Alternaria alternata
(CRL 17),
Curvularia lunata
(CRL 28),
Aspergillus terrus
(CRL 52), and
Aspergillus clavatonanicus
(CRL 22).</description><identifier>ISSN: 0959-3993</identifier><identifier>ISSN: 1573-0972</identifier><identifier>EISSN: 1573-0972</identifier><identifier>DOI: 10.1007/s11274-024-04078-9</identifier><identifier>PMID: 39046545</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Applied Microbiology ; Aspergillus ; Bioactive compounds ; Biochemical tests ; Biochemistry ; Biomedical and Life Sciences ; Biosynthetic Pathways ; Biotechnology ; Catharanthus - microbiology ; Chromatography ; Chromatography, High Pressure Liquid ; Chromatography, Thin Layer ; Curvularia lunata ; Endophytes ; Endophytes - isolation & purification ; Endophytes - metabolism ; Environmental Engineering/Biotechnology ; Fungi ; Fungi - classification ; Fungi - genetics ; Fungi - isolation & purification ; Fungi - metabolism ; High performance liquid chromatography ; Life Sciences ; Liquid chromatography ; Mass Spectrometry ; Mass spectroscopy ; Microbiology ; Molecular ions ; Plant Leaves - microbiology ; Retention time ; SDG3 Good Health and Wellbeing ; Sequence analysis ; Thin layer chromatography ; Vinblastine ; Vinblastine - metabolism</subject><ispartof>World journal of microbiology & biotechnology, 2024-09, Vol.40 (9), p.278, Article 278</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c300t-c24b13b9b25182d889f8243c8a0b723664014dfb912ebcb5b19779bba9f861873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39046545$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khan, Uzma</creatorcontrib><creatorcontrib>Gond, Surendra Kumar</creatorcontrib><title>Detection of vinblastine and related bioactive compounds from culture extracts of endophytic fungi of Catharanthus roseus</title><title>World journal of microbiology & biotechnology</title><addtitle>World J Microbiol Biotechnol</addtitle><addtitle>World J Microbiol Biotechnol</addtitle><description>This study investigates the synthesis of vinblastine by endophytic fungi isolated from leaf of
C. roseus
. A total of 10 endophytic fungi were selected for secretion of vinca alkaloids based on the initial screening by biochemical tests and thin-layer chromatography (TLC). Out of these ten, only four fungal extracts showed positive results for presence of vinblastine at same retention time (10 min.) compared to reference compound on HPLC analysis. The detected concentration of vinblastine was maximum (17 µg/ml) in isolate no. CRL 22 followed by CRL 52, CRL 17 and CRL 28. To validate the presence of vinblastine, ultra-high-performance liquid chromatography coupled with high-resolution accurate mass spectrometry (HRMS) was employed. This analysis confirmed the presence of anhydrovinblastine, a precursor of vinblastine through the detection of molecular ions at m/z 793.4185 in extract of CRL 17. In addition to anhydrovinblastine, the intermediate compounds essential to the biosynthetic pathway of vinblastine were also detected in the extract of CRL 17. These host-origin compounds strongly suggest the presence of a biosynthetic pathway within the endophytic fungus. Based on morphological observation and sequence analysis of the ITS region of rDNA, endophytic fungi were identified as
Alternaria alternata
(CRL 17),
Curvularia lunata
(CRL 28),
Aspergillus terrus
(CRL 52), and
Aspergillus clavatonanicus
(CRL 22).</description><subject>Applied Microbiology</subject><subject>Aspergillus</subject><subject>Bioactive compounds</subject><subject>Biochemical tests</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthetic Pathways</subject><subject>Biotechnology</subject><subject>Catharanthus - microbiology</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromatography, Thin Layer</subject><subject>Curvularia lunata</subject><subject>Endophytes</subject><subject>Endophytes - isolation & purification</subject><subject>Endophytes - metabolism</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Fungi</subject><subject>Fungi - classification</subject><subject>Fungi - genetics</subject><subject>Fungi - isolation & purification</subject><subject>Fungi - metabolism</subject><subject>High performance liquid chromatography</subject><subject>Life Sciences</subject><subject>Liquid chromatography</subject><subject>Mass Spectrometry</subject><subject>Mass spectroscopy</subject><subject>Microbiology</subject><subject>Molecular ions</subject><subject>Plant Leaves - microbiology</subject><subject>Retention time</subject><subject>SDG3 Good Health and Wellbeing</subject><subject>Sequence analysis</subject><subject>Thin layer chromatography</subject><subject>Vinblastine</subject><subject>Vinblastine - metabolism</subject><issn>0959-3993</issn><issn>1573-0972</issn><issn>1573-0972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kbtuFDEYRi1ERJbAC1AgSzQ0Q3ybsV2iDTcpUhqoLdvzT3aiGXvxJWLfHm82gESRwrJkn-_48iH0hpIPlBB5mSllUnSEtSGIVJ1-hja0l7wjWrLnaEN0rzuuNT9HL3O-I6TFNH-BzrkmYuhFv0GHKyjgyxwDjhO-n4NbbC5zAGzDiBMstsCI3Rxtg-4B-7juYw1jxlOKK_Z1KTUBhl8lNSIfJRDGuN8dyuzxVMPtfFzb2rKzyYayqxmnmKHmV-hsskuG14_zBfrx-dP37dfu-ubLt-3H685zQkrnmXCUO-1YTxUbldKTYoJ7ZYmTjA-DIFSMk9OUgfOud1RLqZ2zjRuokvwCvT959yn-rJCLWefsYVlsgFiz4UQJwtQw9A199x96F2sK7XYPVPs9Ko5CdqJ8e0hOMJl9mlebDoYScyzGnIoxrRjzUIzRLfT2UV3dCuPfyJ8mGsBPQG5b4RbSv7Of0P4G5H-aEw</recordid><startdate>20240901</startdate><enddate>20240901</enddate><creator>Khan, Uzma</creator><creator>Gond, Surendra Kumar</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TB</scope><scope>7TK</scope><scope>7U5</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>L7M</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20240901</creationdate><title>Detection of vinblastine and related bioactive compounds from culture extracts of endophytic fungi of Catharanthus roseus</title><author>Khan, Uzma ; Gond, Surendra Kumar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c300t-c24b13b9b25182d889f8243c8a0b723664014dfb912ebcb5b19779bba9f861873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Applied Microbiology</topic><topic>Aspergillus</topic><topic>Bioactive compounds</topic><topic>Biochemical tests</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthetic Pathways</topic><topic>Biotechnology</topic><topic>Catharanthus - microbiology</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Chromatography, Thin Layer</topic><topic>Curvularia lunata</topic><topic>Endophytes</topic><topic>Endophytes - isolation & purification</topic><topic>Endophytes - metabolism</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Fungi</topic><topic>Fungi - classification</topic><topic>Fungi - genetics</topic><topic>Fungi - isolation & purification</topic><topic>Fungi - metabolism</topic><topic>High performance liquid chromatography</topic><topic>Life Sciences</topic><topic>Liquid chromatography</topic><topic>Mass Spectrometry</topic><topic>Mass spectroscopy</topic><topic>Microbiology</topic><topic>Molecular ions</topic><topic>Plant Leaves - microbiology</topic><topic>Retention time</topic><topic>SDG3 Good Health and Wellbeing</topic><topic>Sequence analysis</topic><topic>Thin layer chromatography</topic><topic>Vinblastine</topic><topic>Vinblastine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khan, Uzma</creatorcontrib><creatorcontrib>Gond, Surendra Kumar</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>World journal of microbiology & biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khan, Uzma</au><au>Gond, Surendra Kumar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of vinblastine and related bioactive compounds from culture extracts of endophytic fungi of Catharanthus roseus</atitle><jtitle>World journal of microbiology & biotechnology</jtitle><stitle>World J Microbiol Biotechnol</stitle><addtitle>World J Microbiol Biotechnol</addtitle><date>2024-09-01</date><risdate>2024</risdate><volume>40</volume><issue>9</issue><spage>278</spage><pages>278-</pages><artnum>278</artnum><issn>0959-3993</issn><issn>1573-0972</issn><eissn>1573-0972</eissn><abstract>This study investigates the synthesis of vinblastine by endophytic fungi isolated from leaf of
C. roseus
. A total of 10 endophytic fungi were selected for secretion of vinca alkaloids based on the initial screening by biochemical tests and thin-layer chromatography (TLC). Out of these ten, only four fungal extracts showed positive results for presence of vinblastine at same retention time (10 min.) compared to reference compound on HPLC analysis. The detected concentration of vinblastine was maximum (17 µg/ml) in isolate no. CRL 22 followed by CRL 52, CRL 17 and CRL 28. To validate the presence of vinblastine, ultra-high-performance liquid chromatography coupled with high-resolution accurate mass spectrometry (HRMS) was employed. This analysis confirmed the presence of anhydrovinblastine, a precursor of vinblastine through the detection of molecular ions at m/z 793.4185 in extract of CRL 17. In addition to anhydrovinblastine, the intermediate compounds essential to the biosynthetic pathway of vinblastine were also detected in the extract of CRL 17. These host-origin compounds strongly suggest the presence of a biosynthetic pathway within the endophytic fungus. Based on morphological observation and sequence analysis of the ITS region of rDNA, endophytic fungi were identified as
Alternaria alternata
(CRL 17),
Curvularia lunata
(CRL 28),
Aspergillus terrus
(CRL 52), and
Aspergillus clavatonanicus
(CRL 22).</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>39046545</pmid><doi>10.1007/s11274-024-04078-9</doi><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0959-3993 |
| ispartof | World journal of microbiology & biotechnology, 2024-09, Vol.40 (9), p.278, Article 278 |
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| source | Springer Nature |
| subjects | Applied Microbiology Aspergillus Bioactive compounds Biochemical tests Biochemistry Biomedical and Life Sciences Biosynthetic Pathways Biotechnology Catharanthus - microbiology Chromatography Chromatography, High Pressure Liquid Chromatography, Thin Layer Curvularia lunata Endophytes Endophytes - isolation & purification Endophytes - metabolism Environmental Engineering/Biotechnology Fungi Fungi - classification Fungi - genetics Fungi - isolation & purification Fungi - metabolism High performance liquid chromatography Life Sciences Liquid chromatography Mass Spectrometry Mass spectroscopy Microbiology Molecular ions Plant Leaves - microbiology Retention time SDG3 Good Health and Wellbeing Sequence analysis Thin layer chromatography Vinblastine Vinblastine - metabolism |
| title | Detection of vinblastine and related bioactive compounds from culture extracts of endophytic fungi of Catharanthus roseus |
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