Dietary methionine functions in proliferative zone maintenance and egg production via sams-1 in Caenorhabditis elegans

• Published in: Journal of biochemistry (Tokyo) 2024-11, Vol.176 (5), p.359-367
• Main Authors: Hirota, Keiko, Yamauchi, Rieko, Miyata, Mai, Kojima, Mariko, Kako, Koichiro, Fukamizu, Akiyoshi
• Format: Article
• Language: English
• Subjects: Animals; Caenorhabditis elegans - genetics; Caenorhabditis elegans - metabolism; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; Cell Proliferation; Diet; Fertility; Germ Cells - cytology; Germ Cells - metabolism; Methionine - metabolism; Methionine Adenosyltransferase - genetics; Methionine Adenosyltransferase - metabolism; Mutation; Ovum - metabolism
• ISSN: 0021-924X; 1756-2651; 1756-2651
• DOI: 10.1093/jb/mvae054

The maintenance of germ cells is critical for the prosperity of offspring. The amount of food consumption is known to be closely related to reproduction, i.e. the number of eggs decreases under calorie-restricted conditions in various organisms. Previous studies in Caenorhabditis elegans have reported that calorie restriction reduces the number of eggs and the reduction can be rescued by methionine. However, the effect of methionine on the reproductive process has not been fully understood. In this study, to assess the gonadal function of methionine metabolism, we firstly demonstrated that a depletion in dietary methionine resulted in reduced levels of S-adenosyl-l-methionine (SAM) and S-adenosyl homocysteine in wild-type N2, but not in glp-1 mutants, which possess only a few germ cells. Second, we found no recovery in egg numbers upon methionine administration in SAM synthase (sams)-1 mutants. Furthermore, a reduced number of proliferative zone nuclei exhibited in the sams-1 mutants was not rescued via methionine. Thus, our results have shown that dietary methionine is required for the normal establishment of both the germline progenitor pool and fecundity, mediated by sams-1.