Integrated Analysis of Patient Networks and Plasmid Genomes to Investigate a Regional, Multispecies Outbreak of Carbapenemase-Producing Enterobacterales Carrying Both blaIMP and mcr-9 Genes

• Published in: The Journal of infectious diseases 2024-07, Vol.230 (1), p.e159
• Main Authors: Wan, Yu, Myall, Ashleigh C, Boonyasiri, Adhiratha, Bolt, Frances, Ledda, Alice, Mookerjee, Siddharth, Weiße, Andrea Y, Getino, Maria, Turton, Jane F, Abbas, Hala, Prakapaite, Ruta, Sabnis, Akshay, Abdolrasouli, Alireza, Malpartida-Cardenas, Kenny, Miglietta, Luca, Donaldson, Hugo, Gilchrist, Mark, Hopkins, Katie L, Ellington, Matthew J, Otter, Jonathan A, Larrouy-Maumus, Gerald, Edwards, Andrew M, Rodriguez-Manzano, Jesus, Didelot, Xavier, Barahona, Mauricio, Holmes, Alison H, Jauneikaite, Elita, Davies, Frances
• Format: Article
• Language: English
• Subjects: Adult; Aged; Anti-Bacterial Agents - pharmacology; Bacterial Proteins - genetics; beta-Lactamases - genetics; Carbapenem-Resistant Enterobacteriaceae - genetics; Carbapenem-Resistant Enterobacteriaceae - isolation & purification; Colistin - pharmacology; Disease Outbreaks; Enterobacteriaceae - drug effects; Enterobacteriaceae - genetics; Enterobacteriaceae Infections - epidemiology; Enterobacteriaceae Infections - microbiology; Enterobacteriaceae Infections - transmission; Female; Genome, Bacterial; Humans; London - epidemiology; Male; Microbial Sensitivity Tests; Middle Aged; Phylogeny; Plasmids - genetics
• ISSN: 1537-6613; 1537-6613
• DOI: 10.1093/infdis/jiae019

Carbapenemase-producing Enterobacterales (CPE) are challenging in healthcare, with resistance to multiple classes of antibiotics. This study describes the emergence of imipenemase (IMP)-encoding CPE among diverse Enterobacterales species between 2016 and 2019 across a London regional network. We performed a network analysis of patient pathways, using electronic health records, to identify contacts between IMP-encoding CPE-positive patients. Genomes of IMP-encoding CPE isolates were overlaid with patient contacts to imply potential transmission events. Genomic analysis of 84 Enterobacterales isolates revealed diverse species (predominantly Klebsiella spp, Enterobacter spp, and Escherichia coli); 86% (72 of 84) harbored an IncHI2 plasmid carrying blaIMP and colistin resistance gene mcr-9 (68 of 72). Phylogenetic analysis of IncHI2 plasmids identified 3 lineages showing significant association with patient contacts and movements between 4 hospital sites and across medical specialties, which was missed in initial investigations. Combined, our patient network and plasmid analyses demonstrate an interspecies, plasmid-mediated outbreak of blaIMPCPE, which remained unidentified during standard investigations. With DNA sequencing and multimodal data incorporation, the outbreak investigation approach proposed here provides a framework for real-time identification of key factors causing pathogen spread. Plasmid-level outbreak analysis reveals that resistance spread may be wider than suspected, allowing more interventions to stop transmission within hospital networks.SummaryThis was an investigation, using integrated pathway networks and genomics methods, of the emergence of imipenemase-encoding carbapenemase-producing Enterobacterales among diverse Enterobacterales species between 2016 and 2019 in patients across a London regional hospital network, which was missed on routine investigations.