Induction of osteogenic differentiation by the extracellular matrix of fetal bone tissues and adult cartilage

• Published in: Tissue & cell 2024-10, Vol.90, p.102475, Article 102475
• Main Authors: Negishi, Jun, Tanaka, Dan, Hashimoto, Yoshihide
• Format: Article
• Language: English
• Subjects: Decellularized tissue; Extracellular matrix; Fetal bone tissue
• ISSN: 0040-8166; 1532-3072; 1532-3072
• DOI: 10.1016/j.tice.2024.102475

Decellularized cortical bone powder derived from adult animals has been shown to induce bone remodeling. Furthermore, it is increasingly evident that the extracellular matrix (ECM) within decellularized tissues differs depending on the source tissue and the age of the animal, leading to distinct effects on cells. In this study, we prepared powders from decellularized fetal and adult porcine bone tissues and conducted biological analyses to determine if the decellularized tissue could induce adipose-derived stem cell differentiation. Decellularized fetal tissues and adult cortical bone were converted into powder by cryomilling, but decellularized adult bone marrow and cartilage were not powdered through this process. In vitro assessments revealed that decellularized fetal tissues, decellularized adult cartilage extract, and decellularized fetal cartilage powder can induce osteoblast differentiation. This study suggests that decellularized fetal bone tissues and adult cartilage contain ECM components that can induce osteoblast differentiation. Additionally, it highlights the utility of decellularized fetal cartilage powder for bone reconstruction. •The composition and function of decellularized bone tissue vary depending on the age of the animal.•ECM of fetal bone tissue and adult cartilage has the ability to induce osteogenic differentiation.•Fetal decellularized bone tissue is useful as a powdered material for bone replacement.