Heat-shock transcription factor HsfA8a regulates heat stress response in Sorbus pohuashanensis

• Published in: Planta 2024-09, Vol.260 (3), p.61, Article 61
• Main Authors: Li, Yuyan, Wu, Qianwen, Zhu, Lingyi, Zhang, Ruili, Tong, Boqiang, Wang, Yan, Han, Yi, Lu, Yizeng, Dou, Dequan, Tian, Zhihui, Zheng, Jian, Zhang, Yan
• Format: Article
• Language: English
• Subjects: Agriculture; Amino acids; Arabidopsis - genetics; Arabidopsis - physiology; Arabidopsis thaliana; Biomedical and Life Sciences; Catalase; Courtyards; Ecology; Forestry; Gene expression; Gene Expression Regulation, Plant; Genes; Heat; Heat resistance; Heat Shock Transcription Factors - genetics; Heat Shock Transcription Factors - metabolism; Heat stress; Heat tolerance; Heat-Shock Response - genetics; High temperature; Hot Temperature; Leaves; Life Sciences; Molecular structure; Molecular weight; Original Article; Ornamental plants; Ornamental trees; Peroxidase; Phosphorylation; Plant Leaves - genetics; Plant Leaves - metabolism; Plant Leaves - physiology; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Sciences; Plants, Genetically Modified; Regulatory mechanisms (biology); Sorbus - genetics; Sorbus - metabolism; Sorbus - physiology; Sorbus pohuashanensis; Structure-function relationships; Sunburn; Temperature; Temperature effects; Temperature tolerance; Thermotolerance - genetics; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism
• ISSN: 0032-0935; 1432-2048; 1432-2048
• DOI: 10.1007/s00425-024-04486-z

Main conclusion The SpHsfA8a upregulated expression can induce the expression of multiple heat-tolerance genes, and increase the tolerance of Arabidopsis thaliana to high-temperature stress. Sorbus pohuashanensis is an ornamental tree used in courtyards. However, given its poor thermotolerance, the leaves experience sunburn owing to high temperatures in summer, severely affecting its ornamental value. Heat-shock transcription factors play a critical regulatory role in the plant response to heat stress. To explore the heat-tolerance-related genes of S. pohuashanensis to increase the tree’s high-temperature tolerance, the SpHsfA8a gene was cloned from S. pohuashanensis , and its structure and expression patterns in different tissues and under abiotic stress were analyzed, as well as its function in heat tolerance, was determined via overexpression in Arabidopsis thaliana . The results showed that SpHsfA8a encodes 416 amino acids with a predicted molecular weight of 47.18 kDa and an isoelectric point of 4.63. SpHsfA8a is a hydrophilic protein without a signal peptide and multiple phosphorylation sites. It also contains a typical DNA-binding domain and is similar to MdHsfA8a in Malus domestica and PbHsfA8 in Pyrus bretschneideri . In S. pohuashanensis , SpHsfA8a is highly expressed in the roots and fruits and is strongly induced under high-temperature stress in leaves. The heterologous expression of SpHsfA8a in A. thaliana resulted in a considerably stronger growth status than that of the wild type after 6 h of treatment at 45 °C. Its proline content, catalase and peroxidase activities also significantly increased, indicating that the SpHsfA8a gene increased the tolerance of A. thaliana to high-temperature stress. SpHsfA8a could induce the expression of multiple heat-tolerance genes in A. thaliana , indicating that SpHsfA8a could strengthen the tolerance of A. thaliana to high-temperature stress through a complex regulatory network. The results of this study lay the foundation for further elucidation of the regulatory mechanism of SpHsfA8a in response of S. pohuashanensis to high-temperature stress.