Genetic circuitry controlling Drosophila female germline overgrowth

• Published in: Developmental biology 2024-11, Vol.515, p.160-168
• Main Authors: Zhang, Qian, Li, Le, Zhang, Qi, Zhang, Yang, Yan, Lizhong, Wang, Yanfang, Wang, Yuejia, Zhao, Shaowei
• Format: Article
• Language: English
• Subjects: bam; bantam; bgcn; Drosophila; Hippo pathway; miRNA pathway
• ISSN: 0012-1606; 1095-564X; 1095-564X
• DOI: 10.1016/j.ydbio.2024.07.016

Germ cells mutant for bam or bgcn are locked in a germline stem cell (GSC)-like state, leading to tumor-like overgrowth in Drosophila ovaries. Our previous studies have demonstrated that germline overgrowth in bam mutants can be suppressed by defects in the miRNA pathway but enhanced by a null mutation in hippo. However, the genetic epistasis between the miRNA and Hippo pathways still remains unknown. Here, we determined that the miRNA pathway acts downstream of the Hippo pathway in regulating this process. Germ cells mutant for bam or bgcn and defective in both pathways divide very slowly, phenocopying those defective only in the miRNA pathway. In addition, we found that Yki, a key oncoprotein in the Hippo pathway, promotes the growth of both wild-type germ cells and bam mutant GSC-like cells. Like wild-type GSCs, bam mutant GSC-like cells predominantly stay in the G2 phase. Remarkably, many of those defective in the miRNA pathway are arrested before entering this phase. Furthermore, our studies identified bantam as a critical miRNA promoting germline overgrowth in bam or bgcn mutants. Taken together, these findings establish a genetic circuitry controlling Drosophila female germline overgrowth. [Display omitted] •miRNA pathway works downstream of Hippo pathway to sustain germline overgrowth.•The key oncoprotein Yki, from the Hippo pathway, promotes germline overgrowth.•Loss of dcr-1 arrest many bam mutant germ cells before entering the G2 phase.•Bantam is both necessary and sufficient for promoting germline overgrowth.