Sulforaphane inhibits histone deacetylase causing cell cycle arrest and apoptosis in oral squamous carcinoma cells

Biologic compounds have recently generated interest in cancer chemoprevention. Sulforaphane (SFN), an isothiocyanate from cruciferous vegetables, has profound epigenetic actions. Since epigenetic aetiology is crucial for oral cancer, this study evaluated the role of SFN in oral cancer prevention. Or...

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Published in:Medical journal. Armed Forces India 2024-07, Vol.80 (4), p.412-419
Main Authors: Krishnan, Manu, Saraswathy, Seema, Singh, Sanjana, Saggu, Gursimran Kaur, Kalra, Namita, Agrawala, Paban K., Abraham, Kurian Mathew, Das Toora, Bishamber
Format: Article
Language:English
Subjects:
Apoptosis
Cell cycle
Histone deacetylase inhibition
Oral cancer
Sulforaphane
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Summary:Biologic compounds have recently generated interest in cancer chemoprevention. Sulforaphane (SFN), an isothiocyanate from cruciferous vegetables, has profound epigenetic actions. Since epigenetic aetiology is crucial for oral cancer, this study evaluated the role of SFN in oral cancer prevention. Oral squamous cell carcinoma cells (UPCI-SCC-172) were treated with SFN in three concentrations: 10 μM, 20 μM and 30 μM for two time periods: 24 h and 48 h. MTT assay assessed cell proliferation. Histone deacetylase (HDAC) enzyme activity was colorimetrically estimated in the nuclear extracts. Flow cytometry determined cell cycle stages, reactive oxygen species (ROS) generation and mitochondrial membrane potential (MMP) changes. Extrinsic and intrinsic apoptotic pathways were evaluated from caspase enzyme assays. Cell proliferation and HDAC activity (44% in 24 h and 40% in 48 h) were significantly inhibited (p 
ISSN:0377-1237
DOI:10.1016/j.mjafi.2022.03.005