Antidiabetic and Antioxidant Activities of Indian Bay Leaf (Cinnamomum tamala (Buch.‐Ham.) T. Nees & Eberm.) Essential Oils Collected from Meghalaya

• Published in: Chemistry & biodiversity 2024-11, Vol.21 (11), p.e202400879-n/a
• Main Authors: Kumar Chaudhary, Sushil, Kharlyngdoh, Evanylla, Shukla, Jitendra K., Bhardwaj, Pardeep K., Thorat, Sunil S., Bhowmick, Shovonlal, Sharma, Nanaocha, Kumar Mukherjee, Pulok
• Format: Article
• Language: English
• Subjects: alpha-Amylases - antagonists & inhibitors; alpha-Amylases - metabolism; alpha-Glucosidases - metabolism; Amylases; Antidiabetics; Antioxidants; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Ascorbic acid; Bay leaf; Benzothiazoles - antagonists & inhibitors; Benzothiazoles - chemistry; Biphenyl Compounds - antagonists & inhibitors; Caryophyllene; Cinnamomum - chemistry; Cinnamomum tamala; Diabetes mellitus; Dietary supplements; Essential oil; Essential oils; Eugenol; Gas chromatography; Gas Chromatography-Mass Spectrometry; Germacrene; Glucosidase; Glycoside Hydrolase Inhibitors - chemistry; Glycoside Hydrolase Inhibitors - isolation & purification; Glycoside Hydrolase Inhibitors - pharmacology; Hills; Hypoglycemic Agents - chemistry; Hypoglycemic Agents - isolation & purification; Hypoglycemic Agents - pharmacology; India; Leaves; Mass spectrometry; Mass spectroscopy; Molecular docking; Oils & fats; Oils, Volatile - chemistry; Oils, Volatile - isolation & purification; Oils, Volatile - pharmacology; Picrates - antagonists & inhibitors; Plant Leaves - chemistry; Sulfonic acid; Sulfonic Acids - antagonists & inhibitors; Vitamin E; α-Amylase; α-glucosidase
• ISSN: 1612-1872; 1612-1880; 1612-1880
• DOI: 10.1002/cbdv.202400879

The current work investigates the chemodiversity, in vitro antioxidant, α‐amylase and α‐glucosidase inhibitory potential of Cinnamomum tamala (CT) leaf essential oil (EO) collected from different localities of East Khasi Hills District of Meghalaya, India. Gas chromatography‐mass spectrometry (GC‐MS) analysis of all the extracted leaf essential oils facilitated the identification of several compounds in a variable range along with eugenol as the major component (74.79–95.12 %). CT8 exhibited the highest antioxidant activity (IC50=11.23±0.27 μg/mL for 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) and IC50=21.54±0.37 μg/mL for 2,2’‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS) among all EO evaluated. The results showed that the ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) values for CT8 were 83.26±1.92 μM trolox/g oil and 70.29±1.90 ascorbic acid equivalents (AAE)/g of oil. α‐amylase and α‐glucosidase inhibition were highest in sample CT8 with IC50 values of 3.62±0.42 μg/mL and 16.29±0.32 μg/mL respectively. Caryophyllene, cyclohexene, 1, 5, 5‐trimethyl‐6‐(2‐propenylidene), germacrene D and eugenol showed strong binding potential toward α‐amylase and α‐glucosidase. It concluded that the chemodiversity and antidiabetic potential of C. tamla oil from Khasi Hills have never been studied. It can be taken as a dietary supplement as an antioxidant and antidiabetic to control blood glucose.