Co-localization and co-expression of Olfml3 with Iba1 in brain of mice

• Published in: Journal of neuroimmunology 2024-09, Vol.394, p.578411, Article 578411
• Main Authors: Yadav, Himanshi, Bakshi, Amrita, Anamika, Singh, Vishal, Paul, Prateek, Murugan, N. Arul, Maurya, Shashank Kumar
• Format: Article
• Language: English
• Subjects: Animals; Brain - metabolism; Calcium-Binding Proteins - biosynthesis; Calcium-Binding Proteins - metabolism; Colocalization; Iba1; Lipopolysaccharides - toxicity; LPS; Male; Mice; Mice, Inbred C57BL; Microfilament Proteins - biosynthesis; Microfilament Proteins - metabolism; Microglia; Microglia - metabolism; Molecular Docking Simulation; Neuroinflammation; Neuroinflammatory Diseases - metabolism; Olfml3
• ISSN: 0165-5728; 1872-8421; 1872-8421
• DOI: 10.1016/j.jneuroim.2024.578411

Olfml3 is a microglia-specific protein whose role in neuroinflammation is elusive. In silico analysis was conducted to characterize the Olfml3 protein, followed by molecular docking and MD simulation to check possible interaction with Iba1. Further, expression and co-localization analysis was performed in the LPS-induced neuroinflammatory mice brains. Results suggest that Olfml3 physically interacts with Iba1. Olfml3 and Iba1 expression increases during neuroinflammation in mice brains. Olfml3 was observed to co-localize with Iba1, and the number of Olfml3 and Iba1 dual-positive cells increased in the brain of the neuroinflammatory mice model. Thus, Olfml3 could potentially participate in microglia functions by interacting with Iba1. [Display omitted] •Olfml3 and Iba1 expression increases during neuroinflammation.•Olfml3 interacts with proteins involved in microglial activation and migration.•Olfml3 is co-localized with Iba1, and the number of Iba1-Olfml3 dual-positive cells increases during neuroinflammation.