Genetic diversity analysis of Saccharosydne procerus in Hunan region, China

• Published in: Molecular biology reports 2024-12, Vol.51 (1), p.878, Article 878
• Main Authors: Shan, Lian, Wu, Yu, Ding, Wenbing, He, Hualiang, Xue, Jin, Gao, Qiao, Qiu, Lin, Li, Youzhi
• Format: Article
• Language: English
• Subjects: Animal Anatomy; Animal Biochemistry; Animals; Biomedical and Life Sciences; China; Evolution; Fulgoroidea; Gene polymorphism; Genetic analysis; Genetic diversity; Genetic structure; Genetic Variation - genetics; Genetics, Population - methods; Hemiptera - genetics; Histology; Host plants; Host-parasite interactions; Life Sciences; Microsatellite Repeats - genetics; Morphology; Original Article; Oryza - genetics; Oryza - parasitology; Phylogeny; Polymorphism, Genetic; Population genetics; Population studies; Rice fields; Saccharosydne procerus; Statistical analysis
• ISSN: 0301-4851; 1573-4978; 1573-4978
• DOI: 10.1007/s11033-024-09770-5

Background Saccharosydne procerus serves as a significant alternative host for parasitoids of the important rice pest, rice planthoppers. Rearing S. procerus on the water bamboo plants near rice field can provide a parasitic site for parasitic wasps during the idle period of rice fields, thereby stabilizing the number of parasitoids and suppressing the number of rice planthoppers in the field. However, limited understanding of genetic diversity of S. procerus restricts its application. Therefore, this study aims to analyze the genetic diversity of S. procerus in Hunan region. Methods In this study, 16 geographical populations of the S. procerus from the Hunan region were used. After screening, ISSR primers were employed for polymorphism detection. POPGENE32 software was used for genetic diversity analysis, and UPGMA clustering was applied for statistical analysis of different geographical populations to generate an evolutionary tree. Results Eleven ISSR primers were screened, resulting in the detection of 194 amplification locus, of which 126 were polymorphic. The average percentage of polymorphic locus was 64.95%. The mean Nei’s gene diversity (H) was 0.2475, the mean Shannon’s Information index (I) was 0.3708, and the Genetic diversity index among populations (G st ) was 0.3800. Cluster analysis identified three groups, with most populations concentrated in the second group, indicating no clear genetic structure. This suggests that the 16 populations of S. procerus exhibit high levels of genetic diversity.