Walnut Jug r 1 is Responsible for Primary Sensitization among Patients Suffering Walnut-Hazelnut 2S Albumin Cross-Reactivity

Walnut and hazelnut coallergy is a frequent manifestation in clinical practice whose molecular basis remains unclear. For this purpose, walnut-hazelnut cross-reactivity was evaluated in 20 patients allergic to one or both tree nuts and sensitized to their 2S albumins. Immunoblotting assays showed th...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2024-08, Vol.72 (32), p.18162-18170
Main Authors: Castromil-Benito, Estela S., Betancor, Diana, Parrón-Ballesteros, Jorge, Bueno-Díaz, Cristina, Gutiérrez-Díaz, Gloria, Turnay, Javier, Heras, Manuel de las, Cuesta-Herranz, Javier, Villalba, Mayte, Pastor-Vargas, Carlos
Format: Article
Language:English
Subjects:
2S Albumins, Plant - chemistry
2S Albumins, Plant - immunology
Adolescent
Adult
Aged
Allergens - chemistry
Allergens - immunology
Antigens, Plant - chemistry
Antigens, Plant - immunology
Child
Corylus - chemistry
Corylus - immunology
Cross Reactions
Female
Food Safety and Toxicology
Humans
Immunoglobulin E - immunology
Juglans - chemistry
Juglans - immunology
Male
Middle Aged
Nut Hypersensitivity - immunology
Nuts - chemistry
Nuts - immunology
Plant Proteins - chemistry
Plant Proteins - immunology
Young Adult
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Summary:Walnut and hazelnut coallergy is a frequent manifestation in clinical practice whose molecular basis remains unclear. For this purpose, walnut-hazelnut cross-reactivity was evaluated in 20 patients allergic to one or both tree nuts and sensitized to their 2S albumins. Immunoblotting assays showed that 85% of patients recognized Jug r 1, walnut 2S albumin, which was associated with the development of severe symptoms; 50% of them corecognized hazelnut 2S albumin, Cor a 14. Both allergens were isolated using chromatographic techniques. Inhibition ELISAs revealed that Jug r 1 strongly inhibited the binding of Cor a 14-specific IgE, but Cor a 14 only partially inhibited Jug r 1-specific IgE binding. Our results showed that patients sensitized to walnut/hazelnut 2S albumins were not a homogeneous population. There were patients sensitized to specific epitopes of walnut 2S albumins and patients sensitized to cross-reactive epitopes between walnut and hazelnut, with Jug r 1 being the primary sensitizer.
ISSN:0021-8561
1520-5118
1520-5118
DOI:10.1021/acs.jafc.4c03603