Fungicide resistance in Colletotrichum fructicola and Colletotrichum siamense causing peach anthracnose in China

• Published in: Pesticide biochemistry and physiology 2024-08, Vol.203, p.106006, Article 106006
• Main Authors: Karim, Mohammad Mazharul, Usman, Hafiz Muhammad, Tan, Qin, Hu, Jia-Jie, Fan, Fei, Hussain, Rafakat, Luo, Chao-Xi
• Format: Article
• Language: English
• Subjects: Aminoimidazole Carboxamide - analogs & derivatives; Benzimidazoles - pharmacology; Carbamates - pharmacology; Carbendazim; China; Colletotrichum - drug effects; Colletotrichum - genetics; Colletotrichum fructicola; Colletotrichum gloeosporioides species complex; Colletotrichum siamense; Drug Resistance, Fungal; Fungicide resistance; Fungicides, Industrial - pharmacology; Hydantoins - pharmacology; Peach anthracnose; Plant Diseases - microbiology; Prunus persica - microbiology; Triazoles - pharmacology
• ISSN: 0048-3575; 1095-9939; 1095-9939
• DOI: 10.1016/j.pestbp.2024.106006

Peach is one of the popular and economically important fruit crops in China. Peach cultivation is hampered due to attacks of anthracnose disease, causing significant economic losses. Colletotrichum fructicola and Colletotrichum siamense belong to the Colletotrichum gloeosporioides species complex and are considered major pathogens of peach anthracnose. Application of different groups of fungicides is a routine approach for controlling this disease. However, fungicide resistance is a significant drawback in managing peach anthracnose nowadays. In this study, 39 isolates of C. fructicola and 41 isolates of C. siamense were collected from different locations in various provinces in China. The sensitivity of C. fructicola and C. siamense to some commonly used fungicides, i.e., carbendazim, iprodione, fluopyram, and propiconazole, was determined. All the isolates of C. fructicola collected from Guangdong province showed high resistance to carbendazim, whereas isolates collected from Guizhou province were sensitive. In C. siamense, isolates collected from Hebei province showed moderate resistance, while those from Shandong province were sensitive to carbendazim. On the other hand, all the isolates of C. fructicola and C. siamense showed high resistance to the dicarboximide (DCF) fungicide iprodione and succinate dehydrogenase inhibitor (SDHI) fungicide fluopyram. However, they are all sensitive to the demethylation inhibitor (DMI) fungicide propiconazole. Positive cross-resistance was observed between carbendazim and benomyl as they are members of the same methyl benzimidazole carbamate (MBC) group. While no correlation of sensitivity was observed between different groups of fungicides. No significant differences were found in each fitness parameter between carbendazim-resistant and sensitive isolates in both species. Molecular characterization of the β-tubulin 2 (TUB2) gene revealed that in C. fructicola, the E198A point mutation was the determinant for the high resistance to carbendazim, while the F200Y point mutation was linked with the moderate resistance to carbendazim in C. siamense. Based on the results of this study, DMI fungicides, e.g., propiconazole or prochloraz could be used to control peach anthracnose, especially at locations where the pathogens have already developed the resistance to carbendazim and other fungicides. [Display omitted] •C. fructicola and C. siamense were resistant to iprodione and fluopyram, but sensitive to propiconazole, while