Biodegradation of four polyolefin plastics in superworms (Larvae of Zophobas atratus) and effects on the gut microbiome

• Published in: Journal of hazardous materials 2024-09, Vol.477, p.135381, Article 135381
• Main Authors: Wang, Shuaibing, Yu, Hong, Li, Wei, Song, Enze, Zhao, Zhiguo, Xu, Jing, Gao, Shangkun, Wang, Dandan, Xie, Zhihong
• Format: Article
• Language: English
• Subjects: Gut microbiota; Plastic biodegradation; Polyolefin plastics; Zophobas atratus
• ISSN: 0304-3894; 1873-3336; 1873-3336
• DOI: 10.1016/j.jhazmat.2024.135381

Recent studies have demonstrated superworms (larvae of Zophobas atratus) ability to degrade polyethylene (PE), polystyrene (PS), polyvinyl chloride (PVC), and polypropylene (PP) within their digestive system. This study aimed to compare the ability of superworms to degrade the above four polyolefin plastics over a duration of 30 days. In this study, the degradation rate of PE was the highest, and the final average weight of superworms, as well as the final plastic mass loss consumed by them, significantly increased (73.38 % and 52.33 %, respectively) when PE was fed with wheat bran (1:1 [w/w]). FTIR and TGA indicated the occurrence of oxidation and biodegradation processes in the four polyolefin plastics when exposed to superworms. In addition, the molecular weights (Mw and Mn) of excreted polymer residues decreased by 3.1 % and 2.87 % in PE-fed superworms, suggesting that the depolymerization of PE was not entirely dependent on the gut microbial community. The analysis of the gut microbial communities revealed that the dominant microbial community were different for each type of plastic. The results indicate that the gut microbiome of superworms exhibited remarkable adaptability in degrading various types of plastics, and the intake preferences and efficiency of different plastics are associated with different dominant microbial community species. [Display omitted] •Comparison of the biodegradability of superworms on four polyolefin plastics.•Superworms showed the highest on the degradation rate of polyethylene (PE).•PE degradation was not completely dependent to the gut microbiota.•Dietary conditions determined the intestinal predominance of gut microbiota.