A reporter virus particle seroneutralization assay for tick‐borne encephalitis virus overcomes ELISA limitations

Tick‐borne encephalitis (TBE) virus is the most prevalent tick‐transmitted orthoflavivirus in Europe. Due to the nonspecific nature of its symptoms, TBE is primarily diagnosed by ELISA‐based detection of specific antibodies in the patient serum. However, cross‐reactivity between orthoflaviviruses co...

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Bibliographic Details
Published in:Journal of medical virology 2024-08, Vol.96 (8), p.e29843-n/a
Main Authors: Ackermann‐Gäumann, Rahel, Dentand, Alexis, Lienhard, Reto, Saeed, Mohsan, Speiser, Daniel E., MacDonald, Margaret R., Coste, Alix T., Cagno, Valeria
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies, Neutralizing - blood
Antibodies, Neutralizing - immunology
Antibodies, Viral - blood
Assaying
Biosafety
Cross Reactions
cross‐reactivity
Dengue fever
Disease transmission
Encephalitis
Encephalitis Viruses, Tick-Borne - immunology
Encephalitis, Tick-Borne - diagnosis
Encephalitis, Tick-Borne - virology
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Epidemiology
Humans
Infectivity
Neutralization
Neutralization Tests - methods
orthoflavivirus
reporter virus particle
Sensitivity and Specificity
serology
seroneutralization
TBEV
Tick-borne encephalitis
Vector-borne diseases
Virion - immunology
Viruses
West Nile virus
Zika virus
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Summary:Tick‐borne encephalitis (TBE) virus is the most prevalent tick‐transmitted orthoflavivirus in Europe. Due to the nonspecific nature of its symptoms, TBE is primarily diagnosed by ELISA‐based detection of specific antibodies in the patient serum. However, cross‐reactivity between orthoflaviviruses complicates the diagnosis. Specificity issues may be mitigated by serum neutralization assays (SNT), although the handling of clinically relevant orthoflaviviruses requires biosafety level (BSL) 3 conditions and they have highly divergent viral kinetics and cell tropisms. In the present study, we established a reporter virus particle (RVP)‐based SNT in which the infectivity is measured by luminescence and that can be performed under BSL‐2 conditions. The RVP‐based SNT for TBEV exhibited a highly significant correlation with the traditional virus‐based SNT (R2 = 0.8637, p 
ISSN:0146-6615
1096-9071
1096-9071
DOI:10.1002/jmv.29843