A Comprehensive Systematic Review of the Effects of Photobiomodulation Therapy in Different Light Wavelength Ranges (Blue, Green, Red, and Near-Infrared) on Sperm Cell Characteristics in Vitro and in Vivo

• Published in: Reproductive sciences (Thousand Oaks, Calif.) Calif.), 2024-11, Vol.31 (11), p.3275-3302
• Main Authors: Moradi, Ali, Ghaffari Novin, Marefat, Bayat, Mohammad
• Format: Article
• Language: English
• Subjects: Animals; Embryology; Humans; Infertility, Male - radiotherapy; Infertility: Review; Infrared Rays - therapeutic use; Low-Level Light Therapy - methods; Male; Medicine; Medicine & Public Health; Obstetrics/Perinatology/Midwifery; Reproductive Medicine; Sperm Motility - radiation effects; Spermatozoa - radiation effects
• ISSN: 1933-7191; 1933-7205; 1933-7205
• DOI: 10.1007/s43032-024-01657-x

Around 7% of the male population in the world are entangle with considerable situation which is known as male infertility. Photobiomodulation therapy (PBMT) is the application of low-level laser radiation, that recently used to increase or promote the various cell functions including, proliferation, differentiation, ATP production, gene expressions, regulation of reactive oxygen spices (ROS), and also boost the tissue healing and reduction of inflammation. This systematic review's main idea is a comprehensive appraisal of the literatures on subjects of PBMT consequences in four light ranges wavelength (blue, green, red, near-infrared (NIR)) on sperm cell characteristics, in vitro and in vivo. In this study, PubMed, Google Scholar, and Scopus databases were used for abstracts and full-text scientific papers published from 2003–2023 that reported the application of PBM on sperm cells. Criteria’s for inclusion and exclusion to review were applied. Finally, the studies that matched with our goals were included, classified, and reported in detail. Also, searched studies were subdivided into the effects of four ranges of light irradiation, including the blue light range (400–500 nm), green light range (500–600 nm), red light range (600–780 nm), and NIR light range (780–3000 nm) of laser irradiation on human or animal sperm cells, in situations of in vitro or in vivo. Searches with our keywords results in 137 papers. After primary analysis, some articles were excluded because they were review articles or incomplete and unrelated studies. Finally, we use the 63 articles for this systematic review. Our category tables were based on the light range of irradiation, source of sperm cells (human or animal cells) and being in vitro or in vivo. Six% of publications reported the effects of blue, 10% green, 53% red and 31% NIR, light on sperm cell. In general, most of these studies showed that PBMT exerted a positive effect on the sperm cell motility. The various effects of PBMT in different wavelength ranges, as mentioned in this review, provide more insights for its potential applications in improving sperm characteristics. PBMT as a treatment method has significant effectiveness for treatment of different medical problems. Due to the lack of reporting data in this field, there is a need for future studies to assessment the biochemical and molecular effects of PBMT on sperm cells for the possible application of this treatment to the human sperm cells before the ART proce