Novel bibenzyl compound Ae exhibits anti-agiogenic activity in HUVECs in vitro and zebrafish in vivo

• Published in: Bioorganic & medicinal chemistry 2024-09, Vol.111, p.117866, Article 117866
• Main Authors: Guan, Li, Zhang, Shengjie, Song, Pengfei, Xia, Yanxin, Zheng, Xinle, Li, Weize
• Format: Article
• Language: English
• Subjects: Angiogenesis Inhibitors - chemical synthesis; Angiogenesis Inhibitors - chemistry; Angiogenesis Inhibitors - pharmacology; Animals; Anti-angiogenesis; Anticancer; Bibenzyl; Bibenzyls - chemical synthesis; Bibenzyls - chemistry; Bibenzyls - pharmacology; Cell Movement - drug effects; Cell Proliferation - drug effects; Dose-Response Relationship, Drug; Human Umbilical Vein Endothelial Cells - drug effects; Humans; Molecular Docking Simulation; Molecular Structure; Receptor, TIE-2 - antagonists & inhibitors; Receptor, TIE-2 - metabolism; Structure-Activity Relationship; Transgenic zebrafish model; Vascular Endothelial Growth Factor Receptor-2 - antagonists & inhibitors; Vascular Endothelial Growth Factor Receptor-2 - metabolism; Zebrafish
• ISSN: 0968-0896; 1464-3391; 1464-3391
• DOI: 10.1016/j.bmc.2024.117866

[Display omitted] •Our new synthesized bibenzyl compound Ae had potent anti-angiogenic activity in zebrafish in vivo.•Ae exhibited the obvious inhibitory activity of proliferation, migration, invasion and tube formation in HUVEC cells in vitro.•The anti-angiogenic activity of compound Ae is connected with the ang-2, tek in ANGPT-TEK pathway and the kdr, kdrl signaling axle in VEGF-VEGFR pathway. The inhibition of angiogenesis has been considered as an attractive method for the discovery of potential anti-cancer drugs. Herein, we report our new synthesized bibenzyl compound Ae had potent anti-angiogenic activity(the lowest effective concentration is to 0.62–1.25 μM) in zebrafish in vivo and showed a concentration-dependent inhibition of inter-segmental blood vessels (ISVs) compared to control. Further, Ae exhibited the obvious inhibitory activity of proliferation, migration, invasion and tube formation in HUVEC cells in vitro. Moreover, qRT-PCR analysis revealed that the anti-angiogenic activity of compound Ae is connected with the ang-2, tek in ANGPT-TEK pathway and the kdr, kdrl signaling axle in VEGF-VEGFR pathway. Molecular docking studies revealed that compound Ae had an interaction with the angiopoietin-2 receptor(TEK) and VEGFR2. Additionally, analysis of the ADMET prediction data indicated that compound Ae possessed favorable physicochemical properties, drug-likeness, and synthetic accessibility. In conclusion, compound Ae had remarkable anti-angiogenic activity and could be served as an candidate for cancer therapy.