Towards sustainable water management for Galdieria sulphuraria cultivation

• Published in: The Science of the total environment 2024-11, Vol.950, p.175267, Article 175267
• Main Authors: Carone, M., Malaguti, M., Zanetti, M., Tiraferri, A., Riggio, V.A.
• Format: Article
• Language: English
• Subjects: Centrifugation; Galdieria sulphuraria; Membrane microfiltration; Water reuse
• ISSN: 0048-9697; 1879-1026; 1879-1026
• DOI: 10.1016/j.scitotenv.2024.175267

The red microalga Galdieria sulphuraria has emerged as a promising biotechnological platform for large-scale cultivation and production of high-value compounds, such as the blue pigment phycocyanin. However, a large amount of freshwater and a substantial supply of nutrients challenge both the environmental and the economic sustainability of algal cultivation. Additionally, the extremophilic nature of Galdieria sulphuraria requires cultivation in an acidic culture medium that directly leads to strongly acidic wastewater, which in turn generally exceeds legal limits for industrial wastewater discharge. This research aims to address these challenges, by investigating cultivation water reuse as a strategy to reduce the impacts of Galdieria sulphuraria management. The results indicated that a 25 % water reuse may be easily implemented and showed to be effective at the pilot scale, providing no significant changes in microalgae growth (biomass productivity ~0.21 g L−1 d−1) or in phycocyanin accumulation (~ 10.8 % w/w) after three consecutive cultivation cycles in reused water. Moreover, a single cultivation cycle with water reuse percentages of 71 and 98 %, achieved with membrane filtration and with centrifugation, respectively, was also successful (biomass productivity ~0.24 g L−1 d−1). These findings encourage freshwater reuse implementations in the microalgae sector and support further investigations focusing on coupling cultivation and harvesting in continuous, real-scale configurations. Centrifugation and membrane filtration required substantially different specific electrical energy consumption for water reuse and biomass concentration: in real applications, the former technique would roughly span from 1 to 10 kWh m−3 while the latter is expected to fall within the ample range 0.1–100 kWh m−3, strongly dependent on system size. For this reason, the most suitable separation train should be chosen on a case-by-case basis, considering the prevailing flow rate and the target biomass concentration factor targeted by the separation process. [Display omitted] •Galdieria sulphuraria cultivation necessitates management of acidic wastewater.•Water and nutrient reuse enhance the sustainability of the cultivation process.•25% water reuse maintains biomass productivity (~ 0.21 g L-1 d-1) and phycocyanin accumulation (~10.8% w/w).•High water reuse (71%, 95%) does not affect productivity, equal to 0.24 g L-1 d-1 in a single reuse cycle.•Energy needs of centrifugation and