Porphyrin Production by Corynebacterium diphtheriae Strains from Clinical Isolates

Porphyrins are intermediate metabolites involved in the biosynthesis of vital molecules, including heme, cobalamin, and chlorophyll. Bacterial porphyrins are known to be proinflammatory and are associated with biofilm formation. This study investigated porphyrin production by strains of Corynebacter...

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Bibliographic Details
Published in:Chemistry & biodiversity 2024-12, Vol.21 (12), p.e202401011-n/a
Main Authors: Alves, Gabriela Batista, Costa Marques Calderari, Mônica Regina, Fonseca, Eduardo Nunes, Santos, Louisy Sanches, Mattos‐Guaraldi, Ana Luiza
Format: Article
Language:English
Subjects:
Biofilms
Biosynthesis
Chromatography, High Pressure Liquid
Clinical isolates
Corynebacterium diphtheria
Corynebacterium diphtheriae
Corynebacterium diphtheriae - isolation & purification
Corynebacterium diphtheriae - metabolism
Emission analysis
Emission spectra
Emission spectroscopy
Fluorescence spectroscopy
Humans
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metabolites
Porphyrins
Porphyrins - biosynthesis
Porphyrins - chemistry
Porphyrins - metabolism
Protoporphyrin
Protoporphyrin IX
Qualitative analysis
Spectrometry, Fluorescence
Spectroscopic analysis
Strain analysis
Strains (organisms)
UV/Vis spectroscopy
Virulence
Vitamin B12
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Summary:Porphyrins are intermediate metabolites involved in the biosynthesis of vital molecules, including heme, cobalamin, and chlorophyll. Bacterial porphyrins are known to be proinflammatory and are associated with biofilm formation. This study investigated porphyrin production by strains of Corynebacterium diphtheriae using emission spectroscopy, high‐performance liquid chromatography with fluorescence detection, diode array detector, and mass spectrometry. Emission spectroscopy revealed characteristic porphyrin emission spectra in all strains, with coproporphyrin III predominating. Qualitative analysis by different chromatographic methods identified coproporphyrin III, uroporphyrin I, and protoporphyrin IX in all strains. Quantitative analysis revealed strain‐dependent coproporphyrin III production. Further studies are required to investigate the relationship between porphyrin production and the virulence potential of Corynebacterium diphtheriae.
ISSN:1612-1872
1612-1880
1612-1880
DOI:10.1002/cbdv.202401011