TaRLK2.4, a transgressive expression receptor like kinase, improves powdery mildew resistance in wheat

• Published in: International journal of biological macromolecules 2024-10, Vol.277 (Pt 4), p.134387, Article 134387
• Main Authors: Liu, Xiaoying, Yang, Chenxiao, Dong, Huixuan, Wu, Siqi, Wang, Guangyu, Han, Xinyue, Fan, Baoli, Shang, Yuntao, Dang, Chen, Xie, Chaojie, Wang, Zhenying
• Format: Article
• Language: English
• Subjects: Early response; Powdery mildew; RLK gene family; TaRLK2.4; Transgressive characteristic; Triticum aestivum L
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.134387

Plants form two immune systems, pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI), to combat Blumeria graminis f. sp. tritici (Bgt) infection during the evolutionary process. In PTI, receptor-like kinases (RLKs) play important roles during pathogen infections. Based on our previous reports, there were 280 TaRLKs identified in early response to powdery mildew infection, which were divided into 34 subfamilies in this study. Differences in gene structures, cis-acting elements, and expression levels implied the function diversity of TaRLKs. TaRLK2.4, a member of LRK10L-RLKs subfamily, contained 665 amino acids, and located on the cell membrane. The main objective of this study was to investigate the role of the receptor-like kinase gene TaRLK2.4 in conferring powdery mildew resistance in wheat. Real-time quantitative PCR results indicated that TaRLK2.4 expressed during Bgt infection process, and exhibited a transgressive expression characteristic in disease resistance NILs (BJ-1). To elucidate the function of TaRLK2.4 during Bgt infection, the comprehensive analysis of virus induced gene silence and over-expression demonstrated that TaRLK2.4 promoted powdery mildew resistance positively. In summary, these results contribute to a deeper understanding of the complex and diverse biological functions of RLKs, and provide new genetic resources for wheat molecular breeding. [Display omitted] •Identified 280 wheat TaRLK genes involved in early response to Bgt infection•TaRLK2.4 transgressive expressed in disease resistance NILs BJ-1•Cloning of TaRLK2.4 and demonstrating its significant resistance to Bgt