Acaricide effect of plants from the Brazilian savanna on a population of Rhipicephalus microplus with phenotypic resistance to cypermethrin and trichlorfon

• Published in: Veterinary parasitology 2024-10, Vol.331, p.110281, Article 110281
• Main Authors: Zaldivar, Maykelin Fuentes, Bastianetto, Eduardo, Pereira Filho, Adalberto Alves, Rodrigues, Daniel Sobreira, Martins Júnior, Valdo Soares, Morais-Costa, Francielle, Vasconcelos, Viviane Oliveira, Duarte, Eduardo Robson, Araujo, Ricardo Nascimento
• Format: Article
• Language: English
• Subjects: Acaricide resistance; Chromatography; Cypermethrin; Flavonoids; Plant extracts; Tannin; Trichlorfon
• ISSN: 0304-4017; 1873-2550; 1873-2550
• DOI: 10.1016/j.vetpar.2024.110281

Rhipicephalus microplus is among the most important ectoparasites for livestock. The use of synthetic acaricides has raised some concerns due to the selection of tick populations that are resistant to acaricides and environmental contamination. Therefore, plant extracts have been used as alternatives for the treatment of animals infested with ticks. In this study, R. microplus populations from seven different dairy farms were collected and assessed for their resistance to the acaricides cypermethrin or trichlorfon. Larvae of the most resistant population were used in assays to evaluate the acaricide effect of leaf extracts from plants of the Brazilian savanna. The most active extracts were also tested against fully engorged females. Among seven tick populations, five and three showed resistance level ≥ III for cypermethrin or trichlorfon, respectively. The most resistant tick population was evaluated in mortality assays with the plants Piptadenia viridiflora, Annona crassiflora, Caryocar brasiliense, Ximenia americana, and Schinopsis brasilienses. The ethanolic extracts of C. brasiliense, X. americana and S. brasilienses showed higher larvicidal effects in comparison to the other extracts and cypermethrin. The ethanolic extract of X. americana showed 60.79 % efficacy against fully engorged females of the acaricide resistant tick strain. The ethanolic extracts of C. brasiliense, X. americana, and S. brasilienses showed peaks in HPLC-DAD, indicating the presence of tannins and flavonoids. Three of the plants showed promising results and should be explored in further studies to develop novel tools to control R. microplus in cattle. [Display omitted] •Acaricide resistant R. microplus are commonly found in dairy farms.•Plants extracts induce mortality on acaricide resistant R. microplus larvae.•Plants extracts are effective against acaricide resistant R. microplus females.•Plant extracts are an alternative to control acaricide resistant ticks.