Influence of Some Heterocyclic, Cyclic, and Nitrogen-Containing Compounds on Oxidative Deamination of Polyamines in a Cell-Free Test System

• Published in: Bulletin of experimental biology and medicine 2024-07, Vol.177 (3), p.307-312
• Main Authors: Syatkin, S. P., Blagonravov, M. L., Hilal, A., Sungrapova, K. Yu, Sokuev, R. I., Korzun, I. A., Goryachev, V. A.
• Format: Article
• Language: English
• Subjects: Amine Oxidase (Copper-Containing) - metabolism; Animals; Benzoic acid; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cell-Free System; Deamination; Hepatocytes; Heterocyclic Compounds - chemistry; Heterocyclic Compounds - pharmacology; Internal Medicine; Laboratory Medicine; Liver - drug effects; Liver - metabolism; Male; Nitrogen; Nitrogen - metabolism; Oxidation-Reduction - drug effects; Oxidoreductases Acting on CH-NH Group Donors - metabolism; Pathology; Polyamine Oxidase; Polyamines; Polyamines - metabolism; Putrescine; Putrescine - metabolism; Putrescine - pharmacology; Rats; Rats, Wistar; Spermidine; Spermidine - metabolism; Spermine; Spermine - metabolism; Spermine - pharmacology; Test systems
• ISSN: 0007-4888; 1573-8221; 1573-8221
• DOI: 10.1007/s10517-024-06179-9

We studied the effects of some nitrogen-containing, heterocyclic, and cyclic compounds on the rate of oxidative deamination of polyamines and putrescine in tissues with a high proliferation rate. For this purpose, the specific activities of the main enzymes of polyamine oxidative degradation — spermine oxidase (SMO), polyamine oxidase (PAO), and diamine oxidase (DAO) were determined using a cell-free test system from regenerating rat liver. The compounds methyl 2-(5-formylfuran-2-yl)benzoate and 2,7-bis-[2-(diethylamino)ethoxy]-9H-fluoren-9-one (and in the form of dihydrochloride) showed mainly activating effect on oxidative degradation of putrescine, spermidine, and spermine, which indirectly indicates their antiproliferative effect. Nitrogen-free compounds inhibited this process, thus exhibiting potentially carcinogenic properties. Correlations were calculated for activity of DAO, PAO, and SMO with 5 topological indices: Wiener (W), Rouvray (R), Balaban (J) in the Trinaistich modification, detour (Ip), and electropy (Ie). The highest dependence was noted for DAO and the Balaban index ( R =-0.55), for PAO and the detour index ( R =0.78), and for SMO and the electropy index ( R =0.53). The remaining dependencies showed insignificant correlation strength.