Biological S0 reduction at neutral and acidic conditions: Performance and microbial community shifts in a H2/CO2-fed bioreactor

• Published in: Water research (Oxford) 2024-10, Vol.263, p.122156, Article 122156
• Main Authors: Hidalgo-Ulloa, Adrian, van der Graaf, Charlotte M., Sánchez-Andrea, Irene, Weijma, Jan, Buisman, Cees J.N.
• Format: Article
• Language: English
• Subjects: Biological resource recovery; Elemental sulfur reduction; Evaluation process conditions; Microbial diversity changes; Microbial sulfur reduction
• ISSN: 0043-1354; 1879-2448; 1879-2448
• DOI: 10.1016/j.watres.2024.122156

•Acetogenesis enables heterotrophic sulfur reduction at neutral pH.•The diversity of microbial sulfur-reducing genera decreased upon transition to acidic pH.•Desulfurella was the dominant sulfur-reducing genus at acidic conditions.•Sulfidogenic rates suggest intrinsic limits to microbial reduction of elemental sulfur. Sulfidogenesis is a promising technology for the selective recovery of chalcophile bulk metals (e.g. Cu, Zn, and Co) from metal-contaminated waters such as acid mine drainage (AMD) and metallurgy waste streams. The use of elemental sulfur (S0) instead of sulfate (SO42−) as electron acceptor reduces electron donor requirements four-fold, lowering process costs, and expanding the range of operating conditions to a more acidic pH. We previously reported autotrophic S0 reduction using an industrial mesophilic granular sludge as inoculum under thermoacidophilic conditions. Here, we examined the effect of pH on the S0 reduction performance of the same inoculum, in a gas-lift reactor run at 30°C under neutral (pH 6.9) and acidic (pH 3.8) conditions, continuously fed with mineral media and H2 and CO2. Steady-state volumetric sulfide production rates (VSPR) dropped 2.5-fold upon transition to acidic pH, from 1.79 ± 0.18 g S2−·L−1·d−1 to 0.71 ± 0.07 g S2−·L−1·d−1. Microbial community composition was analyzed using 16S rRNA gene amplicon sequencing. At neutral pH (6.9), the high relative abundance of the S0-reducing genus Sulfurospirillum, previously known only for heterotrophic members, combined with the presence of Acetobacterium and detection of acetate, suggests an important role for heterotrophic S0 reduction facilitated by acetogenesis. Conversely, at acidic pH (3.9), S0 reduction appeared autotrophic, as indicated by the high relative abundance of Desulfurella. [Display omitted]