Rapid Revealing of Quorum Sensing (QS)-Regulated PLA, Biofilm and Lysine Targets of Lactiplantibacillus plantarum L3

Quorum sensing (QS) can regulate the production of multiple functional factors in bacteria, but the process of identifying its regulatory targets is very complex and labor-intensive. In this study, an efficient and rapid method to find QS targets through prediction was used. The genome of Lactiplant...

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Bibliographic Details
Published in:Current microbiology 2024-10, Vol.81 (10), p.303, Article 303
Main Authors: Chai, Yunmei, Ma, Qingwen, He, Jinze, Wei, Guangqiang, Huang, Aixiang
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biofilms
Biofilms - growth & development
Biomedical and Life Sciences
Biotechnology
Carbon-Sulfur Lyases
Gene Expression Regulation, Bacterial
Gene regulation
Genes
Genome, Bacterial
Genomes
Lactiplantibacillus plantarum
Lactobacillus plantarum - genetics
Lactobacillus plantarum - metabolism
Lactobacillus plantarum - physiology
Life Sciences
LuxS protein
Lysine
Lysine - metabolism
Metabolic pathways
Microbiology
Original Paper
Predictions
Quorum Sensing
Real time
Regulation
Strings
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Summary:Quorum sensing (QS) can regulate the production of multiple functional factors in bacteria, but the process of identifying its regulatory targets is very complex and labor-intensive. In this study, an efficient and rapid method to find QS targets through prediction was used. The genome of Lactiplantibacillus plantarum ( L. plantarum ) L3 was sequenced and characterized, and then linked the L. plantarum L3 genome to the STRING database for QS system regulatory target prediction. A total of 3,167,484 base pairs (bps) were examined from the genome of L. plantarum L3, and 30 QS-related genes were discovered (including luxS ). The STRING database prediction indicated that the 30 QS-related genes are mainly involved in the regulation of nine metabolic pathways. Furthermore, metE , metK , aroB , cysE , and birA1 were predicted to be regulatory targets of the LuxS/AI-2 QS system, and these five targets were validated based on quantitative real-time PCR and content determination. Successful elucidation of the LuxS/AI-2 QS system’s key targets and regulation mechanism in L. plantarum L3 demonstrated the effectiveness of the new approach for predicting QS targets and provides a scientific basis for future work on improving regulation of functional factor production.
ISSN:0343-8651
1432-0991
1432-0991
DOI:10.1007/s00284-024-03834-2