Establishment of highly metastatic sublines and insights into telomerase expression during tumor metastasis using a microfluidic system

• Published in: Talanta (Oxford) 2024-12, Vol.280, p.126690, Article 126690
• Main Authors: Wang, Jie, Xu, Jiali, Liu, Xiaopeng, Tong, Yuxiao, Xu, Zhangrun
• Format: Article
• Language: English
• Subjects: Cell Line, Tumor; Cell Movement; Cell Proliferation; Cell screening; Epithelial-Mesenchymal Transition; Gene Expression Regulation, Neoplastic; Humans; Lab-On-A-Chip Devices; Microfluidic Analytical Techniques - instrumentation; Microfluidic chip; Neoplasm Metastasis; Signal Transduction; Telomerase; Telomerase - genetics; Telomerase - metabolism; Transcriptome sequencing; Tumor metastasis
• ISSN: 0039-9140; 1873-3573; 1873-3573
• DOI: 10.1016/j.talanta.2024.126690

Metastasis is an important hallmark of malignant tumors, and telomerase often exhibits high expression in these tumors. Monitoring the real-time dynamics of telomerase will provide valuable insights into its association with tumor metastasis. In this study, we described a microfluidic system for screening highly metastatic sublines based on differential cell invasiveness, investigated telomerase expression in the process of tumor metastasis and explored the genes and signaling pathways involved in tumor metastasis. Cells with different metastasis abilities were efficiently classified into different channels, and the fluorescence imaging visually demonstrates that cells with higher metastasis ability have stronger telomerase activity. In addition, we successfully established the high-metastasis-ability LoVo subline (named as LoVo-H) and low-metastasis-ability LoVo subline (named as LoVo-L) from the human colorectal cancer LoVo cell lines through only one round of selection using the system. The results show that the LoVo-H cells display superior proliferation and invasiveness compared to LoVo-L cells. Furthermore, 6776 differentially expressed genes of LoVo-H compared with LoVo-L were identified by transcriptome sequencing. The genes associated with telomerase activity, cell migration and the epithelial to mesenchymal transition were up-regulated in LoVo-H, and PI3K-Akt signaling pathway, extracellular matrix-receptor interaction and Rap1 signaling pathway were significantly enriched in LoVo-H. This microfluidic system is a highly effective tool for selecting highly metastatic sublines and the LoVo-H subline established through this system presents a promising model for tumor metastasis research. Furthermore, this work preliminarily reveals telomerase expression during tumor metastasis and provides a new strategy for studying tumor metastasis and cancer diagnosis. [Display omitted] •Cells screening and telomerase detection were accomplished in one chip.•Compared to parent cells, highly metastatic sublines show stronger telomerase activity.•The highly invasive subline LoVo-H was isolated in one round of screening on the chip.•The transcriptome of LoVo-H exhibits significant changes compared to parent cells.