Sparassis latifolia polysaccharide alleviated lipid metabolism abnormalities in kidney of lead-exposed mice by regulating oxidative stress-mediated inflammation and autophagy based on multi-omics

• Published in: International journal of biological macromolecules 2024-10, Vol.278 (Pt 1), p.134662, Article 134662
• Main Authors: Lu, Xingru, Wu, Shanshan, Ai, Honghu, Wu, Rui, Cheng, Yanfen, Yun, Shaojun, Chang, Mingchang, Liu, Jingyu, Meng, Junlong, Cheng, Feier, Feng, Cuiping, Cao, Jinling
• Format: Article
• Language: English
• Subjects: Animals; Autophagy - drug effects; Inflammation - drug therapy; Inflammation - metabolism; Kidney - drug effects; Kidney - metabolism; Kidney - pathology; Lead; Lead - toxicity; Lipid metabolism; Lipid Metabolism - drug effects; Lipidomics; Male; Mice; Multiomics; Oxidative stress; Oxidative Stress - drug effects; Polysaccharides - pharmacology; Sparassis latifolia polysaccharide; Transcriptomics
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.134662

Lead is a common environmental pollutant which can accumulate in the kidney and cause renal injury. However, regulatory effects and mechanisms of Sparassis latifolia polysaccharide (SLP) on lipid metabolism abnormality in kidney exposed to lead are not clarified. In this study, mice were used to construct an animal model to observe the histopathological changes in kidney, measure lead content, damage indicators, differentially expressed metabolites (DEMs) and genes (DEGs) in key signaling pathways that cause lipid metabolism abnormalities based on lipidomics and transcriptomics, which were later validated using qPCR and western blotting. Co-treatment of Pb and N-acetylcysteine (NAC) were used to verify the link between SLP and oxidative stress. Our results indicated that treatment with SLP identified 276 DEMs (including metabolism of glycerophospholipid, sphingolipid, glycerolipid and fatty acid) and 177 DEGs (including genes related to oxidative stress, inflammation, autophagy and lipid metabolism). Notably, regulatory effects of SLP on abnormal lipid metabolism in kidney were mainly associated with oxidative stress, inflammation and autophagy; SLP could regulate abnormal lipid metabolism in kidney by reducing oxidative stress and affecting its downstream-regulated autophagy and inflammatory to alleviate renal injury caused by lead exposure. This study provides a theoretical basis for SLP intervention in lead injury. •Sparassis latifolia Polysaccharide (SLP) reduced the lead-induced damage of kidney.•SLP treatment obtained 276 identifiable DEMs and 177 identifiable DEGs.•SLP modulated metabolism of glycerophospholipid, glycerolipid, fatty acid, etc.•SLP reduced oxidative stress and regulated its mediated inflammation and autophagy.•SLP regulated lipid metabolism by oxidative stress-mediated inflammation/autophagy.