Enhancing reductive dechlorination of trichloroethylene in bioelectrochemical systems with conductive materials

• Published in: Environmental research 2024-11, Vol.261, p.119773, Article 119773
• Main Authors: Chen, Su-Hao, Li, Zheng-Tao, Lai, Chun-Yu, Zhao, He-Ping
• Format: Article
• Language: English
• Subjects: Bioelectrochemical systems; Charcoal - chemistry; Conductive materials; Direct electron transfer; Electrochemical Techniques - methods; Electrodes; Halogenation; Magnetite Nanoparticles - chemistry; Oxidation-Reduction; Reductive dechlorination; Trichloroethene; Trichloroethylene - chemistry; Trichloroethylene - metabolism; Water Pollutants, Chemical - chemistry
• ISSN: 0013-9351; 1096-0953; 1096-0953
• DOI: 10.1016/j.envres.2024.119773

The incorporation of conductive materials to enhance electron transfer in bioelectrochemical systems (BES) is considered a promising approach. However, the specific effects and mechanisms of these materials on trichloroethylene (TCE) reductive dechlorination in BES remains are not fully understood. This study investigated the use of magnetite nanoparticles (MNP) and biochars (BC) as coatings on biocathodes for TCE reduction. Results demonstrated that the average dechlorination rates of MNP-Biocathode (122.89 μM Cl·d−1) and BC-Biocathode (102.88 μM Cl·d−1) were greatly higher than that of Biocathode (78.17 μM Cl·d−1). Based on MATLAB calculation, the dechlorination rate exhibited a more significantly increase in TCE-to-DCE step than the other dechlorination steps. Microbial community analyses revealed an increase in the relative abundance of electroactive and dechlorinating populations (e.g., Pseudomonas, Geobacter, and Desulfovibrio) in MNP-Biocathode and BC-Biocathode. Functional gene analysis via RT-qPCR showed the expression of dehalogenase (RDase) and direct electron transfer (DET) related genes was upregulated with the addition of MNP and BC. These findings suggest that conductive materials might accelerate reductive dechlorination by enhancing DET. The difference of physicochemical characteristics (e.g. particle size and specific surface area), electron transfer enhancement mechanism between MNP and BC as well as the reduction of Fe(III) by hydrogen may explain the superior dechlorination rate observed with MNP-Biocathode. [Display omitted] •Conductive materials accelerate reductive dechlorination rate by enhancing direct electron transfer.•Conductive materials promote the proportion of electroactive and dechlorinating microorganisms.•The manifold differences between magnetite nanoparticles and biochars can explain the higher dechlorination rate in magnetite nanoparticles-biocathode.