Deletions of ttrA and pduA genes in Salmonella enterica affect survival within chicken-derived HD-11 macrophages

• Published in: Current genetics 2024-12, Vol.70 (1), p.14
• Main Authors: Saraiva, Mauro M. S., Benevides, Valdinete P., Guerra, Priscila R., Campos, Isabella C., Rodrigues Alves, Lucas B., Paiva, Jacqueline B., Muniz, Lauanda M., Almeida, Adriana M., Freitas Neto, Oliveiro C., Olsen, John E., Berchieri Junior, Angelo
• Format: Article
• Language: English
• Subjects: Anaerobic microorganisms; Anaerobic processes; Anaerobic respiration; Animals; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biochemistry; Biomedical and Life Sciences; Cell Biology; Cell Line; Cell lines; Chickens; Chickens - microbiology; Clonal deletion; Energy metabolism; Fermentation; Gene Deletion; Intestine; Life Sciences; Lysis; Macrophages; Macrophages - immunology; Macrophages - metabolism; Macrophages - microbiology; Microbial Genetics and Genomics; Microbial Viability - genetics; Microbiology; Microorganisms; Original Article; Plant Sciences; Proteomics; Salmonella; Salmonella enterica; Salmonella enterica - genetics; Salmonella Infections, Animal - immunology; Salmonella Infections, Animal - microbiology; Survival; Tetrathionate
• ISSN: 0172-8083; 1432-0983; 1432-0983
• DOI: 10.1007/s00294-024-01299-1

In mammals, enteric salmonellas can use tetrathionate ( ttr ), formed as a by-product from the inflammatory process in the intestine, as electron acceptor in anaerobic respiration, and it can fuel its energy metabolism by degrading the microbial fermentation product 1,2-propanediol. However, recent studies have shown that this mechanism is not important for Salmonella infection in the intestine of poultry, while it prolongs the persistence of Salmonella at systemic sites in this species. In the current study, we show that Δ ttr A pdu A strains of Salmonella enterica have lower net survival within chicken-derived HD-11 macrophages, as CFU was only 2.3% ( S. Enteritidis Δ ttr A pdu A), 2.3% ( S. Heidelberg Δ ttr A pdu A), and 3.0% ( S . Typhimurium Δ ttr A pdu A) compared to wild-type strains after 24 h inside HD-11 macrophage cells. The difference was not related to increased lysis of macrophages, and deletion of ttr A and pdu A did not impair the ability of the strains to grow anaerobically. Further studies are indicated to determine the reason why Salmonella Δ ttr A pdu A strains survive less well inside macrophage cell lines. Graphical abstract