Identification of human circulating factors following remote ischemic conditioning (RIC): Potential impact on stroke

Remote ischemic conditioning (RIC) is a procedure consisting of short cycles of ischemia applied in a limb that activates endogenous protection in distant organs, such as the brain. Despite the promising outcomes of RIC, the biochemical factors governing inter-organ communication remain largely unex...

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Published in:Free radical biology & medicine 2024-11, Vol.224, p.23-38
Main Authors: Mollet, Inês G., Viana-Soares, Ricardo, Cardoso-Pires, Catarina, Soares, Nuno L., Marto, João Pedro, Mendonça, Marcelo, Queiroga, Cláudia S.F., Carvalho, Ana S., Sequeira, Catarina O., Teixeira-Santos, Luísa, Fernandes, Tatiana P., Aloria, Kerman, Pereira, Sofia A., Matthiesen, Rune, Viana-Baptista, Miguel, Vieira, Helena L.A.
Format: Article
Language:English
Subjects:
Adult
Biomarkers
Female
Glutathione - blood
Glutathione - metabolism
Humans
Ischemic Preconditioning - methods
Male
Middle Aged
Neuroinflammation
Nitric Oxide - blood
Nitric Oxide - metabolism
Oxidative stress
Pilot Projects
Proteomics
Proteomics - methods
Remote ischemic (pre)conditioning
Stroke
Stroke - blood
Stroke - metabolism
Young Adult
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Summary:Remote ischemic conditioning (RIC) is a procedure consisting of short cycles of ischemia applied in a limb that activates endogenous protection in distant organs, such as the brain. Despite the promising outcomes of RIC, the biochemical factors governing inter-organ communication remain largely unexplored, particularly in humans. A pilot study on 20 healthy humans was performed to identify potential circulating biochemical factors involved in RIC signalling. Blood was collected before and immediately, 4 and 22 h after the end of RIC. To characterize the responses triggered by RIC, a combination of biochemical and proteomic analysis, along with functional in vitro tests in human cells, were performed. RIC did not alter the levels of nitric oxide, bilirubin and cell-free mitochondrial DNA. In contrast, carboxyhaemoglobin levels increased following RIC at all time points and young subset, suggesting endogenous production of carbon monoxide that is a cytoprotective gasotransmitter. Additionally, the levels of glutathione and cysteinylglycine bound to proteins also increased after RIC, while glutathione catabolism decreased. Plasma proteomic analysis identified overall 828 proteins. Several steps of statistical analysis (Student's t-test, repeated measures ANOVA, with Holm corrected pairwise p-values
ISSN:0891-5849
1873-4596
1873-4596
DOI:10.1016/j.freeradbiomed.2024.08.017