Pectin from steam explosion-treated citrus peel exhibits good emulsion properties and bioavailability-promoting effect in vitro of nobiletin

• Published in: International journal of biological macromolecules 2024-10, Vol.278 (Pt 2), p.134758, Article 134758
• Main Authors: Yang, Xiaohan, Liu, Xiaohan, Zhao, Shaojie, Huo, Man, Tian, Guifang, Sang, Yaxin
• Format: Article
• Language: English
• Subjects: Bioavailability in vitro; Biological Availability; Caco-2 Cells; Citrus - chemistry; Citrus pectin; Emulsions - chemistry; Emulsying properties; Flavones - chemistry; Flavones - pharmacokinetics; Flavones - pharmacology; Humans; Nobiletin; Pectins - chemistry; Pectins - pharmacology; Steam; Steam explosion
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.134758

Steam explosion (SE) is a potential method to modify pectin structure, which might be connected to its emulsifying characteristics and the bioavailability of encapsulated polymethoxyflavone like nobiletin. However, the relationship between SE-modified pectin and the bioavailability of encapsulated nobiletin is still unclear. In this study, nobiletin-loaded emulsion was fabricated using citrus pectin modified with SE (0.15–0.9 MPa, 3 min) as emulsifier for in vitro digestion study, and the transport and absorption of nobiletin in Caco-2 cells to investigate the bioavailability-promoting effect. The results showed that SE treatment lowered the droplet size of emulsion from 21.38 ± 2.30 μm to 2.14 ± 0.12 μm, enhanced the nobiletin encapsulation efficiency from 23.73 ± 0.78% to 86.27 ± 3.81%, improved the nobiletin bioaccessibility in vitro from 2.48 ± 0.10% to 25.42 ± 0.10% and increased the intracellular accumulation of nobiletin by over 10 times, even higher than that of Tween 80. In conclusion, pectin from SE-treated citrus peel exhibited good emulsion properties and bioavailability-promoting effect in vitro of nobiletin. [Display omitted] •The extraction rate of pectin after steam explosion increased from 9.29 % to 30.65 %.•Pectin treated at 0.3 and 0.45 MPa had good emulsion stability and loading rate.•Pectin showed quick absorption rate with high linearity and GalA but low DM and Mw.•Emulsion stabled by pectin treated at 0.45 MPa had optimal nobiletin bioavailability.