Microplastics at an environmentally relevant dose enhance mercury toxicity in a marine copepod under multigenerational exposure: Multi-omics perspective

• Published in: Journal of hazardous materials 2024-10, Vol.478, p.135529, Article 135529
• Main Authors: Bai, Zhuoan, He, Yaohui, Hu, Guosheng, Cheng, Luman, Wang, Minghua
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis - drug effects; Copepoda - drug effects; Mercury - toxicity; Mercury pollution; Microplastics; Microplastics - toxicity; Molecular mechanism; Multi-omics; Multigenerational exposure; Multiomics; Proteomics; Reproduction - drug effects; Water Pollutants, Chemical - toxicity
• ISSN: 0304-3894; 1873-3336; 1873-3336
• DOI: 10.1016/j.jhazmat.2024.135529

Here, we subjected the marine copepod Tigriopus japonicus to environmentally-relevant concentrations of microplastics (MPs) and mercury (Hg) for three generations (F0–F2) to investigate their physiological and molecular responses. Hg accumulation and phenotypic traits were measured in each generation, with multi-omics analysis conducted in F2. The results showed that MPs insignificantly impacted the copepod’s development and reproduction, however, which were significantly compromised by Hg exposure. Interestingly, MPs significantly increased Hg accumulation and consequently aggravated this metal toxicity in T. japonicus, demonstrating their carrier role. Multi-omics analysis indicated that Hg pollution produced numerous toxic events, e.g., induction of apoptosis, damage to cell/organ morphogenesis, and disordered energy metabolism, ultimately resulting in retarded development and decreased fecundity. Importantly, MPs enhanced Hg toxicity mainly via increased oxidative apoptosis, compromised cell/organ morphogenesis, and energy depletion. Additionally, phosphoproteomic analysis revealed extensive regulation of the above processes, and also impaired neuron activity under combined MPs and Hg exposure. These alterations adversely affected development and reproduction of T. japonicus. Overall, our findings should offer novel molecular insights into the response of T. japonicus to long-term exposure to MPs and Hg, with a particular emphasis on the carrier role of MPs on Hg toxicity. [Display omitted] •Copepod’s multigenerational exposure to MPs and Hg was performed.•MPs insignificantly impacted the copepod’s development and reproduction.•MPs increased Hg toxicity to the growth/reproduction in copepods.•Hg-induced compromised traits were linked to energy depletion and high apoptosis.•MPs enhanced Hg toxicity by high oxidative apoptosis and impaired neuron activity.