Toxicological assays in the evaluation of safety assessment of fibrinolytic enzymes

• Published in: Drug and chemical toxicology (New York, N.Y. 1978) N.Y. 1978), 2024-11, Vol.47 (6), p.1393-1403
• Main Authors: Marques da Silva, Marllyn, Santana Moura, Yanara Alessandra, Leite, Arthur Hipólito Pereira, Souza, Kerolayne Lira Da Silva, Brandão Costa, Romero Marcos Pedrosa, Nascimento, Thiago Pajeú, Porto, Ana Lúcia Figueiredo, Bezerra, Raquel Pedrosa
• Format: Article
• Language: English
• Subjects: Animals; Drug Evaluation, Preclinical; Fibrinolysis - drug effects; Fibrinolytic Agents - toxicity; Humans; Peptide Hydrolases - metabolism; Toxicity Tests
• ISSN: 0148-0545; 1525-6014; 1525-6014
• DOI: 10.1080/01480545.2024.2367561

Cardiovascular diseases (CVDs) cause 30% of deaths each year, and in 2030, around 23.6 million people will die due to CVDs. The major challenge is to obtain molecules with minimal adverse reactions that can prevent and dissolve blood clots. In this context, fibrinolytic enzymes from diverse microorganism sources have been extensively investigated due to their potential to act directly and specifically on the fibrin clot, preventing side effects and performing potential thrombolytic effects. However, most researches focus on the purification and characterization of proteases, with little emphasis on the mechanism of action and pharmacological characteristics, including toxicity assays which are essential to assess safety and side effects. Therefore, this work aims to emphasize the importance of evaluations indicating the toxicological profile of fibrinolytic proteases through in vitro and in vivo tests. Both types of assays contribute as preclinical stage in drug development and are crucial for clinical applications. This scarcity creates arbitrary barriers to further studies. This work should further encourage the development of studies to ensure the safety and effectivity of fibrinolytic proteases.