Nanoparticles encapsulated in Abelmoschus esculentus polysaccharide-based pellets as colon targeting approach

• Published in: Journal of microencapsulation 2024-10, Vol.41 (7), p.1-534
• Main Authors: Arora, Akshita, Sharma, Anshul, Singh, Shamsher, Singh, Rajveer, Singh, Amrinder, Kakkar, Dipti, Sharma, Nitin
• Format: Article
• Language: English
• ISSN: 0265-2048; 1464-5246; 1464-5246
• DOI: 10.1080/02652048.2024.2390951

This article explores the application of mesalazine-loaded nanoparticles (MLZ-NPs) encapsulated in plant polysaccharide-based pellets (MLZ-NPs-Pellets) for ulcerative colitis. MLZ-NPs were prepared and evaluated for diameter, PDI, and entrapment efficiency. efficacy study was conducted on Caco-2 cells. MLZ-NPs were encapsulated in polysaccharides to form MLZ-NPs-Pellets and characterised for efficacy in animals and targeting efficiency in human volunteers. Optimised batch of MLZ-NPs were characterised for diameter, PDI, zeta potential and entrapment efficiency which was found to be 145.42 ± 6.75 nm, 0.214 ± 0.049, -31.63 mV and 77.65 ± 2.33(%w/w) respectively. ROS, superoxide and NF-kβ were well controlled in Caco-2 cells when treated with MLZ-NPs. data revealed that some parameters (body weight, colon length, lipid peroxidase, and glutathione) recovered significantly in the DSS-induced mice model treated with oral MLZ-NPs-Pellets. Gamma scintigraphy revealed that the formulation can effectively target the colon within 600 min. MLZ-NPs-Pellets can be effectively used for microbial-triggered colon targeting approach in treating ulcerative colitis.