Sequencing, assembly, and genomic annotation of Leucoagaricus gongylophorus LEU18496, a dikarya mutualistic species

• Published in: Molecular omics 2024-09, Vol.20 (8), p.524-531
• Main Authors: Castillo-Alfonso, Freddy, Valadez-Cano, Cecilio, Cejas-Añón, Gabriela, Utrilla, José, Sigala Alanis, Juan-Carlos, Le Borgne, Sylvie, Sales-Cruz, Alfonso Mauricio, Vigueras-Ramírez, Gabriel, Olivares-Hernández, Roberto
• Format: Article
• Language: English
• ISSN: 2515-4184; 2515-4184
• DOI: 10.1039/d4mo00108g

The basidiomycete fungus is able to grow in the fungus garden of leaf-cutter ants. This mutualistic interaction has driven the evolutionary adaptation of , shaping its metabolism to produce enzymes adept at lignocellulosic biomass degradation. In this study, we undertook the comprehensive sequencing, assembly, and functional annotation of the genome of strain LEU18496, mutualistic fungus of the . Our genomic analyses revealed a distinctive bimodal nature to the genome: a predominant region characterized by AT enrichment and low genetic density, alongside a smaller region exhibiting higher GC content and higher genetic density. The presence of transposable elements (TEs) within the AT-enriched region suggests genomic compartmentalization, facilitating differential evolutionary rates. With a gene count of 6748, the assembled genome of LEU18496 surpasses previous reports for this fungal species. Inspection of genes associated with central metabolism unveiled a remarkable abundance of carbohydrate-active enzymes (CAZymes) and fungal oxidative lignin enzymes (FOLymes), underscoring their pivotal roles in the life cycle of this fungus.