Insights into mitochondrial creatine kinase: examining preventive role of creatine supplement in doxorubicin-induced cardiotoxicity

• Published in: Toxicology mechanisms and methods 2024-08, p.1
• Main Authors: Sharif, Salaheddin M, Hydock, David
• Format: Article
• Language: English
• ISSN: 1537-6524; 1537-6524
• DOI: 10.1080/15376516.2024.2393825

Doxorubicin (Dox) is an effective and commonly used anticancer drug; however, it leads to several side effects including cardiotoxicity which contributes to poor quality of life for cancer patients. Creatine (Cr) is a promising intervention to alleviate Dox-induced cardiotoxicity. This study aimed to examine the effects of Cr beforeDox on cardiac mitochondrial creatine kinase (MtCK). Male rats were randomly assigned to one of two 4-week Cr feeding interventions (standard Cr diet or Cr loading diet) or a control diet (Con,  = 20). Rats in the standard Cr diet (Cr1,  = 20) were fed 2% Cr for 4-weeks. Rats in the Cr loading diet (Cr2,  = 20) were fed 4% Cr for 1-week followed by 2% Cr for 3-weeks. After 4-weeks, rats received either a bolus injection of 15 mg/kg Dox or a placebo saline injection (Sal). Five days post-injections left ventricle (LV) was excised and analyzed for MtCK expression using Western blot and ELISA. A significant drug effect was observed for LV mass (