Effect of Ca2+ signal on the activity of key enzymes of carbon metabolism and related gene expression in yeast under high sugar fermentation

BACKGROUND Saccharomyces cerevisiae is a fungus widely used in the food industry and biofuel industry, whereas it is usually exposed to high sugar stress during the fermentation process. Ca2+ is a key second messenger of the cell, it can regulate cell metabolism. The present study investigated the e...

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Bibliographic Details
Published in:Journal of the science of food and agriculture 2025-01, Vol.105 (1), p.276-284
Main Authors: Xie, Dongdong, Zheng, Jiaxin, Sun, Yingqi, Li, Xing, Ren, Shuncheng
Format: Article
Language:English
Subjects:
Biofuels
Calcium ions
Calcium metabolism
Calcium phosphates
calcium signal
Calcium signalling
Carbon
carbon metabolism
Enzymes
Fermentation
Fermented food
Flow resistance
Food industry
Food processing
Gene expression
Gene flow
Genes
genes expression
Glucose
Glucose metabolism
Glucose transport
Glycerol
Glycolysis
Hexokinase
high sugar fermentation
Metabolism
Pentose
Pentose phosphate pathway
Saccharomyces cerevisiae
Sugar
Trehalose
Yeast
Yeasts
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Summary:BACKGROUND Saccharomyces cerevisiae is a fungus widely used in the food industry and biofuel industry, whereas it is usually exposed to high sugar stress during the fermentation process. Ca2+ is a key second messenger of the cell, it can regulate cell metabolism. The present study investigated the effect of the Ca2+ signal on the activity of key enzymes of carbon metabolism and related gene expression in yeast under high sugar fermentation. RESULTS The expression of genes encoding hexokinase was up‐regulated in the high sugar environment, the activity of hexokinase was increased, glucose transmembrane transport capacity was enhanced, the ability of glucose to enter into glycolytic metabolism was increased, and the expression of genes related to pentose phosphate metabolism, glycerol metabolism and trehalose metabolism was up‐regulated in the high glucose with Ca2+ group. CONCLUSION Ca2+ signal regulates the cellular metabolism of glycerol and trehalose and optimizes the allocation of carbon flow by regulating the key enzymes and related gene expression to enhance the resistance of yeast to high sugar stress. © 2024 Society of Chemical Industry.
ISSN:0022-5142
1097-0010
1097-0010
DOI:10.1002/jsfa.13826