MEMBRANE PROTEIN 1 encoding an amino acid transporter confers resistance to blast fungus and leaf-blight bacterium in rice

• Published in: Journal of experimental botany 2024-12, Vol.75 (22), p.7284
• Main Authors: Jiang, Tingting, Huang, Ning, Wang, Zhixue, Li, Jiawen, Ma, Lu, Wang, Xinying, Shen, Lingtong, Zhang, Ying, Yu, Yao, Wang, Wei, Fan, Yunxin, Liu, Kunquan, Zhao, Zhi, Xiong, Ziwei, Song, Qisheng, Tang, Haijuan, Zhang, Hongsheng, Bao, Yongmei
• Format: Article
• Language: English
• Subjects: Amino Acid Transport Systems - genetics; Amino Acid Transport Systems - metabolism; Animals; Ascomycota; Disease Resistance - genetics; Genome-Wide Association Study; Oryza - genetics; Oryza - immunology; Oryza - metabolism; Oryza - microbiology; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Proteins - genetics; Plant Proteins - metabolism; Quantitative Trait Loci; Xanthomonas - physiology
• ISSN: 0022-0957; 1460-2431; 1460-2431
• DOI: 10.1093/jxb/erae350

Amino acid transporters (AATs) have been shown to be involved in immune responses during plant-pathogen interactions; however, the molecular mechanism by which they function in this process remains unclear. Here, we used a joint analysis of a genome-wide association study and quantitative trait locus (QTL) mapping to identify MEMBRANE PROTEIN 1, which acts as a QTL in rice against blast fungus. Heterogeneous expression of OsMP1 in yeast supported its function in transporting a wide range of amino acids, including Thr, Ser, Phe, His, and Glu. OsMP1 could also mediate 15N-Glu efflux and influx in Xenopus oocyte cells. The expression of OsMP1 was significantly induced by Magnaporthe oryzae in the resistant rice landrace Heikezijing, whereas no such induction was observed in the susceptible landrace Suyunuo. Overexpressing OsMP1 in Suyunuo enhanced disease resistance to blast fungus and leaf blight bacterium without resulting in a yield penalty. In addition, the overexpression of OsMP1 led to increased accumulation of Thr, Ser, Phe, and His in the leaves and this contributed to the reduced disease susceptibility, which was associated with up-regulation of the jasmonic acid pathway. Our results demonstrate the important role of OsMP1 in disease resistance in rice and provide a potential target for breeding more resistant cultivars without reducing yield.