Fluorescent probes for neuroscience: imaging ex vivo brain tissue sections

Neurobiological research relies heavily on imaging techniques, such as fluorescence microscopy, to understand neurological function and disease processes. However, the number and variety of fluorescent probes available for tissue section imaging limits the advance of research in the field. In this r...

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Bibliographic Details
Published in:Analyst (London) 2024-09, Vol.149 (18), p.4536-4552
Main Authors: Schwehr, Bradley J, Hartnell, David, Ellison, Gaewyn, Hindes, Madison T, Milford, Breah, Dallerba, Elena, Hickey, Shane M, Pfeffer, Frederick M, Brooks, Doug A, Massi, Massimiliano, Hackett, Mark J
Format: Article
Language:English
Subjects:
Animals
Availability
Brain
Brain - diagnostic imaging
Fluorescent Dyes - chemistry
Fluorescent indicators
Humans
Imaging techniques
Medical imaging
Microscopy, Fluorescence - methods
Neurosciences - methods
Organelles
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Summary:Neurobiological research relies heavily on imaging techniques, such as fluorescence microscopy, to understand neurological function and disease processes. However, the number and variety of fluorescent probes available for tissue section imaging limits the advance of research in the field. In this review, we outline the current range of fluorescent probes that are available to researchers for brain section imaging, including their physical and chemical characteristics, staining targets, and examples of discoveries for which they have been used. This review is organised into sections based on the biological target of the probe, including subcellular organelles, chemical species ( , labile metal ions), and pathological phenomenon ( , degenerating cells, aggregated proteins). We hope to inspire further development in this field, given the considerable benefits to be gained by the greater availability of suitably sensitive probes that have specificity for important brain tissue targets.
ISSN:0003-2654
1364-5528
1364-5528
DOI:10.1039/d4an00663a