Effects of polysaccharide from Pueraria lobata on osteoarthritis in rats

• Published in: International journal of biological macromolecules 2024-10, Vol.278 (Pt 3), p.134901, Article 134901
• Main Authors: Zhao, Shifan, Tao, Yijiong, Huang, Linjie, Xue, Hualei, Chen, Rong, Li, Xiao, Chen, Kai, Tang, Qi, Wang, Mi
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Apoptosis - drug effects; Chondrocytes - drug effects; Chondrocytes - metabolism; Cytokines - metabolism; Disease Models, Animal; Lipopolysaccharides; Male; Osteoarthritis; Osteoarthritis - drug therapy; Osteoarthritis - pathology; Polysaccharide; Polysaccharides - chemistry; Polysaccharides - pharmacology; Pueraria - chemistry; Pueraria lobata; Rats; Rats, Sprague-Dawley; Synoviocytes - drug effects; Synoviocytes - metabolism; Synoviocytes - pathology
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.134901

The effects of Pueraria lobata polysaccharide (PPL-1) on osteoarthritis (OA) disease were comprehensively evaluated by using chondrocytes and synoviocytes extracted from the joints of SD rats based on in vitro cell experiments and by establishing pathological models of OA rats. The results showed that concentrations of 1.25–10 and 0.2–1.6 μg/mL, PPL-1 did not inhibit or promote chondrocytes and synoviocytes in vitro. However, at concentrations of 1.25–10 and 0.2–1.6 μg/mL, it can promote cartilage and synovial membrane cells after LPS stimulation of cell activity and inhibite LPS-induced apoptosis. The results of animal experiments showed that PPL-1 can reduce the symptoms of joint swelling in OA rats, decrease the production of serum inflammatory cytokines TNF-α, IL-1β, and IL-6, and slow down the occurrence of inflammation. Therefore, from the perspective of symptoms, inflammatory factors and pathology, PPL-1 has therapeutic effects on OA rats and alleviates the development of inflammation. It indicated that PPL-1 has the potential to be developed into an OA therapeutic drug with anti-inflammatory properties that protects and activates chondrocytes.