Comparative Evaluation of Micellization and Cellular Uptake of β‑Carotene Affected by Flavonoids

Based on in vitro digestion, micellar synthesis, and Caco-2 cell model, this study investigated the effects of typical flavonoids in citrus (naringenin, naringin, hesperetin, hesperidin, quercetin, and rutin) at different doses on the micellization and cellular uptake of β-carotene. In in vitro dige...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2024-09, Vol.72 (35), p.19353-19365
Main Authors: Xu, Yang, Zhang, Nawei, Shi, Kaixin, Zhang, PeiPei, Xiong, Sihui, Xu, Gang, Pan, Siyi
Format: Article
Language:English
Subjects:
beta Carotene - chemistry
beta Carotene - metabolism
Bioactive Constituents, Metabolites, and Functions
bioavailability
Biological Transport
Caco-2 Cells
Citrus
Citrus - chemistry
Citrus - metabolism
class
Digestion
dispersibility
Flavanones - chemistry
Flavanones - metabolism
Flavonoids - chemistry
Flavonoids - metabolism
food chemistry
hesperetin
hesperidin
human cell lines
Humans
intestines
Membrane Transport Proteins
Micelles
naringenin
naringin
Plant Extracts - chemistry
Plant Extracts - metabolism
quercetin
rutin
Rutin - chemistry
Rutin - metabolism
solubility
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Summary:Based on in vitro digestion, micellar synthesis, and Caco-2 cell model, this study investigated the effects of typical flavonoids in citrus (naringenin, naringin, hesperetin, hesperidin, quercetin, and rutin) at different doses on the micellization and cellular uptake of β-carotene. In in vitro digestion, low-dose flavonoids enhanced β-carotene bioaccesssibility by regulating the stability and dispersibility of the intestinal medium, particularly quercetin, which significantly increased the bioaccessibility by 44.6% (p < 0.05). Furthermore, naringenin, hesperetin, hesperidin, and quercetin enhanced the micellar incorporation rate of β-carotene; however, naringin and rutin exhibited an opposite effect, particularly naringin, which significantly reduced it by 71.3% (p < 0.05). This phenomenon could be attributed to the high solubility of naringin and rutin in micelles, resulting in a competitive inhibitory effect on β-carotene. Besides, all treatments significantly enhanced β-carotene cellular uptake (p < 0.05) by promoting the expression of scavenger receptor class B type I and Niemann-Pick C1-Like 1.
ISSN:0021-8561
1520-5118
1520-5118
DOI:10.1021/acs.jafc.4c03554