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Enhanced pH-mediated stacking of anions for CE incorporating a dynamic pH junction

A technique has been developed to enhance analyte focusing for CE for the analysis of physiological samples. High‐ionic‐strength samples are titrated to low‐ionic‐strength on‐line using pH‐mediated sample stacking in conjunction with a dynamic pH junction. This method concentrates analytes by reduci...

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Bibliographic Details
Published in:Electrophoresis 2007-10, Vol.28 (20), p.3786-3793
Main Authors: Arnett, Stacy D., Lunte, Craig E.
Format: Article
Language:English
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Summary:A technique has been developed to enhance analyte focusing for CE for the analysis of physiological samples. High‐ionic‐strength samples are titrated to low‐ionic‐strength on‐line using pH‐mediated sample stacking in conjunction with a dynamic pH junction. This method concentrates analytes by reducing their electrophoretic mobility during field‐amplification. Parameters responsible for enhanced focusing were investigated, and an enhanced pH‐mediated stacking method was optimized for anionic nucleosides. The process results in ultra‐narrow peak widths, for example, 0.28 s for thymidine with a 10 min analysis time. Peak width and resolution with the enhanced stacking method were also compared to normal base stacking and electrokinetic injection. With this technique, mass‐loading capacity can be increased without degradation in peak shape and resolution is dramatically improved.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200700215