Identification of highly polar nitroaromatic compounds in leachate and ground water samples from a TNT-contaminated waste site by LC-MS, LC-NMR, and off-line NMR and MS investigations

• Published in: Analytical and bioanalytical chemistry 2007-11, Vol.389 (6), p.1979-1988
• Main Authors: Preiss, Alfred, Elend, Manfred, Gerling, Susanne, Berger-Preiss, Edith, Steinbach, Klaus
• Format: Article
• Language: English
• Subjects: Chromatography, Liquid - methods; Germany; Hazardous Waste - analysis; Hydrocarbons, Aromatic - analysis; Hydrocarbons, Aromatic - chemistry; Industrial Waste; Magnetic Resonance Spectroscopy - methods; Mass Spectrometry - methods; Nitro Compounds - analysis; Nitro Compounds - chemistry; Polar nitroaromatic compounds; Spectroscopic data; TNT-contaminated waste site; Water Pollutants, Chemical - analysis; Water Supply - analysis
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-007-1573-8

Leachate and ground water samples from a trinitrotoluene-contaminated waste disposal site near a former ammunitions plant in Stadtallendorf, Germany, were analyzed by liquid chromatography (LC)-mass spectrometry (MS) and LC-NMR hyphenated techniques to comprehensively characterize the range of highly polar nitroaromatic compounds. Wherever unknown components could not be identified by comparison with a multistandard, the spectroscopic data obtained on-line were used to make initial structure proposals, which were later confirmed by comparison with authentic reference materials. In those cases where reference materials were not commercially available, unknown compounds were isolated by HPLC cuts and their structures were elucidated by off-line NMR and MS investigations. A variety of previously unknown compounds, including nitrophenols, nitrobenzyl alcohols, methylnitrobenzoic acids, and hydroxynitrobenzoic acids, could be identified. The NMR and MS data are presented here. The main polar compounds were additionally quantified.