Maternal immune activation accelerates pup reflex development and alters immune proteins in pup stomach contents and brain

• Published in: Brain research 2024-12, Vol.1845, p.149198, Article 149198
• Main Authors: Trim, Michael J., Wheeler, Ryan V., Franklin, Tamara B.
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn; Brain - immunology; Brain - metabolism; CD-1; Chemokines; Cytokines; Cytokines - metabolism; Disease Models, Animal; Female; Lactocrine; Lipopolysaccharides; Male; Maternal immune activation; Mice; Poly I-C - pharmacology; Pregnancy; Prenatal Exposure Delayed Effects - immunology; Reflex - physiology; Sensorimotor development; Stomach - immunology
• ISSN: 0006-8993; 1872-6240; 1872-6240
• DOI: 10.1016/j.brainres.2024.149198

•Viral/bacterial maternal immune activation (MIA) models cause accelerated maturation.•Pregnant dam activity is reduced immediately after MIA but this resolves within 24 h.•Bacterial MIA results in lower levels of IL-13 detected in pup stomach contents.•Bacterial MIA results in lower levels of IL-4 detected in pup brain. Prenatal infection increases the risk for neurodevelopmental disorders including autism spectrum disorder and schizophrenia. To better understand this link, a number of maternal immune activation (MIA) rodent models have been studied. However, the majority of these studies focus on adult behavioural outcomes that mirror adult symptoms related to neurodevelopmental disorders. There is little research reporting the effects of MIA on early postnatal development and even fewer using outbred mouse strains. Here, we use a modified version of the Fox scale to assess the effects of two MIA models, a bacterial model (LPS) and a viral model (PolyIC), on overall mouse pup sensorimotor development in CD-1 mice. Surprisingly, both bacterial and viral MIA models resulted in early reflex development when compared with control pups. To better characterize potential factors related to these changes, we examined indicators of sickness/inflammation in the immune-activated dams and in their pups. Sickness behaviour in the dams resulting from immune activation was assessed using a telemetry implant that allowed for continuous recording of temperature and activity in dams exposed to bacterial or viral immune activation. Although MIA dams showed reduced activity on the day immediately following MIA compared to controls, there was no evidence of fever. All dams showed elevated cytokines/chemokines associated with parturition, but this resolved by day 10 post-parturition and was unaffected by previous immune activation. Although circulating cytokines/chemokines in the dams were similar across MIA treatments, there were differences in the amount of interleukin-12p70 and interleukin-13 present in milk taken from milk bands in MIA pups, and interleukin-4 was overall decreased in LPS pup brain. These findings demonstrate that bacterial and viral models of MIA can result in similar precocious development in mice but differing long-term effects on inflammatory markers in both the milk provided to the pups and in their brains.