Teeth with vital pulps and stage III periodontitis unresponsive to therapy exhibit a pulpal inflammatory profile similar to symptomatic irreversible pulpitis

• Published in: International endodontic journal 2024-12, Vol.57 (12), p.1769-1782
• Main Authors: Louzada, Lidiane Mendes, Arruda‐Vasconcelos, Rodrigo, Kearney, Michaela, Yamauchi, Yukako, Gomes, Brenda P. F. A., Duncan, Henry F.
• Format: Article
• Language: English
• Subjects: Adult; Biomarkers; Biomarkers - analysis; Biomarkers - metabolism; Decision making; Dental pulp; Dental Pulp - metabolism; Endodontics; endodontic‐periodontal lesion; Female; gene expression; Gum disease; Humans; Inflammation; Interleukin-10 - metabolism; Interleukin-1beta - metabolism; irreversible pulpitis; Male; MicroRNAs; MicroRNAs - metabolism; Middle Aged; miRNA; Patients; Periodontal diseases; Periodontitis; Periodontitis - metabolism; Periodontitis - therapy; Pulpitis - metabolism; Root Canal Therapy - methods; Root canals; Teeth; Tumor necrosis factor; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-TNF; Young Adult
• ISSN: 0143-2885; 1365-2591; 1365-2591
• DOI: 10.1111/iej.14139

Aim The aim of this study is to investigate the expression of inflammatory biomarkers (TNF‐α, IL‐10, IL‐1β) and the pulpitis‐associated miRNA (miR‐30a‐5p and miR‐128‐3p) in pulp tissue samples from unrestored teeth with a vital normal pulp (NP), teeth with symptomatic irreversible pulpitis (IP) and in unrestored teeth with periodontal disease, unresponsive to periodontal therapy, and a vital pulp (EP). Methodology Thirty patients were included in this observational study (10 teeth with NP, 10 teeth with IP, 10 teeth with EP). Dental pulp tissues samples were collected from patients during root canal treatment (RCT). RNA was extracted and qRT‐PCR of target genes (tumour necrosis factor [TNF]‐α, interleukin [IL]‐1β, IL‐10) and miRNAs (has‐miR‐30a‐5p, has‐miR‐128‐3p) performed to assess the expression profile. Fold‐change in expression was calculated using the formula 2−(ΔCt(Exp)−ΔCt(Ctrl)). One‐way anova with post‐hoc Tukey's was used to determine significant differences between groups. The significance level was set at 5% (p