The novel MFS efflux pump SxtP, regulated by the LysR-type transcriptional activator SxtR, is involved in the susceptibility to sulfamethoxazole/trimethoprim (SXT) and the pathogenesis of Acinetobacter baumannii

• Published in: Antimicrobial agents and chemotherapy 2024-10, Vol.68 (10), p.e0071224
• Main Authors: Gaona, Marc, Corral, Jordi, Campoy, Susana, Barbé, Jordi, Pérez Varela, María, Aranda, Jesús
• Format: Article
• Language: English
• Subjects: Acinetobacter baumannii - drug effects; Acinetobacter baumannii - genetics; Acinetobacter baumannii - metabolism; Acinetobacter baumannii - pathogenicity; Acinetobacter Infections - drug therapy; Acinetobacter Infections - microbiology; Animals; Anti-Bacterial Agents - pharmacology; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Drug Resistance, Multiple, Bacterial - genetics; Gene Expression Regulation, Bacterial; Mechanisms of Resistance; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; Mice; Microbial Pathogenesis; Microbial Sensitivity Tests; Transcription Factors - genetics; Transcription Factors - metabolism; Trimethoprim, Sulfamethoxazole Drug Combination - pharmacology; Virulence - genetics
• ISSN: 0066-4804; 1098-6596; 1098-6596
• DOI: 10.1128/aac.00712-24

is a notorious opportunistic pathogen responsible for healthcare-associated infections worldwide. Efflux pumps play crucial roles in mediating antimicrobial resistance, motility, and virulence. In this study, we present the identification and characterization of the new efflux pump SxtP belonging to the MFS superfamily ( ajor acilitator uperfamily), along with its associated activator ysR type ranscriptional egulator (LTTR) SxtR, demonstrating their roles in sulfamethoxazole/trimethoprim (also known as co-trimoxazole or SXT) resistance, surface-associated motility and virulence.