Exploring antiviral effect and mechanism of Jinye Baidu granules（JYBD）against influenza A virus through network pharmacology and in vitro and invivo experiments

• Published in: Journal of ethnopharmacology 2025-01, Vol.336, p.118720, Article 118720
• Main Authors: Chen, Zhixuan, Mo, Qinxian, Luo, Siqi, Liang, Jinlong, Li, Yinyan, Gao, Yinhuang, Zhang, Chunyu, Jiang, Linrui, Ma, Jun, Yang, Sizu, Jiang, Feng, Liu, Menghua, Liu, Shuwen, Yang, Jie
• Format: Article
• Language: English
• Subjects: Chemical profile; Inflammatory response; Influenza A virus; Jinye Baidu granules; Potential mechanism
• ISSN: 0378-8741; 1872-7573; 1872-7573
• DOI: 10.1016/j.jep.2024.118720

Jinye Baidu granules (JYBD) have been used to treat acute respiratory tract infections and demonstrated clinical efficacy for the treatment of emerging or epidemic respiratory viruses such as SARS-CoV-2 and influenza virus. This study is to investigate the antiviral effect of JYBD against influenza A viruses (IAV) in vitro and in vivo and elucidate its underlying mechanism. Ultra-high-performance liquid chromatography connected with Orbitrap mass spectrometer (UHPLC-Orbitrap MS) was employed to describe the chemical profile of JYBD. The potential pathways and targets involved in JYBD against IAV infection were predicted by network pharmacology. The efficacy and mechanism of JYBD were validated through both in vivo and in vitro experiments. Moreover, combination therapy with JYBD and the classic anti-influenza drugs was also investigated. A total of 126 compounds were identified by UHPLC-Orbitrap MS, of which 9 compounds were unambiguously confirmed with reference standards. JYBD could significantly inhibit the replication of multiple strains of IAV, especially oseltamivir-resistant strains. The results of qRT-PCR and WB demonstrated that JYBD could inhibit the excessive induction of pro-inflammatory cytokines induced by IAV infection and regulate inflammatory response through inhibiting JAK/STAT, NF-κB and MAPK pathways. Moreover, both JYBD monotherapy or in combination with oseltamivir could alleviate IAV-induced severe lung injury in mice. JYBD could inhibit IAV replication and mitigate virus-induced excessive inflammatory response. Combinations of JYBD and neuraminidase inhibitors conferred synergistic suppression of IAV both in vitro and in vivo. It might provide a scientific basis for clinical applications of JYBD against influenza virus infected diseases. [Display omitted] •A total of 126 compounds of Jinye Baidu granules (JYBD) were identified by UHPLC-Orbitrap MS.•The mechanism of JYBD against influenza A virus (IAV) infection was predicted by network pharmacology analysis.•JYBD inhibits JAK/STAT, NF-κB, and MAPK pathways to attenuate influenza A virus-mediated inflammation.•Combinations of JYBD and neuraminidase inhibitors conferred synergistic antiviral effects both in vitro and in vivo.