Transformation of maltose into prebiotic isomaltooligosaccharides by a novel α-glucosidase from Xantophyllomyces dendrorhous

• Published in: Process biochemistry (1991) 2007-11, Vol.42 (11), p.1530-1536
• Main Authors: Fernández-Arrojo, Lucía, Marín, Dolores, Gómez De Segura, Aranzazu, Linde, Dolores, Alcalde, Miguel, Gutiérrez-Alonso, Patricia, Ghazi, Iraj, Plou, Francisco J., Fernández-Lobato, María, Ballesteros, Antonio
• Format: Article
• Language: English
• Subjects: Alpha-glucosidase; Glycosidase; Isomaltooligosaccharides prebiotics; Oligosaccharides; Transglycosylation
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2007.08.007

The transglycosylation activity of a novel α-glucosidase from the basidiomycetous yeast Xanthophyllomyces dendrorhous (formerly Phaffia rhodozyma) was studied using maltose as glucosyl donor. The enzyme synthesized oligosaccharides with α-(1 → 2), α-(1 → 4) and α-(1 → 6) bonds. Using 200 g/l maltose, the yield of oligosaccharides was 53.8 g/l, with prebiotic oligosaccharides containing at least one α-(1 → 6) linkage (panose, 6- O-α-glucosyl-maltotriose and 6- O-α-isomaltosyl-maltose) being the major products (47.1 g/l). The transglycosylatying yield was 3.6 times higher than the observed with the α-glucosidase from Saccharomyces cerevisiae (53.8 vs. 14.7 g/l). Moreover, when increasing the maltose concentration up to 525 g/l, the maximum production of tri- and tetrasaccharides reached 167.1 g/l, without altering the percentage of oligosaccharides in the mixture. Compared with other microbial α-glucosidases in which the main transglycosylation product is a disaccharide, the enzyme from X. dendrorhous yields a final product enriched in trisaccharides and tetrasaccharides.