Photodynamic therapy on mRNA levels in bacteria

Antimicrobial photodynamic therapy (aPDT) has shown efficacy in inactivating different bacterial species by photosensitizer-induced free radical production. Despite aPDT is considered unable to cause resistant strains, enzymatic pathways for detoxification of reactive oxygen species and transmembran...

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Bibliographic Details
Published in:Lasers in medical science 2024-08, Vol.39 (1), p.229, Article 229
Main Authors: Pires, Bruno Ricardo Barreto, de Paoli, Flavia, Mencalha, Andre Luiz, de Souza da Fonseca, Adenilson
Format: Article
Language:English
Subjects:
Bacteria
Bacteria - drug effects
Bacteria - genetics
Dentistry
Detoxification
Dyes
Effectiveness
Efflux
Evaluation
Free radicals
Gene expression
Gram-positive bacteria
Human tissues
Humans
Lasers
Medicine
Medicine & Public Health
Natural products
Optical Devices
Optics
Photochemotherapy - methods
Photodynamic therapy
Photonics
Photosensitizing Agents - pharmacology
Photosensitizing Agents - therapeutic use
Polymerase chain reaction
Proteins
Quantum Optics
Radiation
Reactive oxygen species
Reverse Transcriptase Polymerase Chain Reaction
Reverse transcription
Review Article
RNA, Bacterial - analysis
RNA, Messenger - genetics
RNA, Messenger - metabolism
Spectrum analysis
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Summary:Antimicrobial photodynamic therapy (aPDT) has shown efficacy in inactivating different bacterial species by photosensitizer-induced free radical production. Despite aPDT is considered unable to cause resistant strains, enzymatic pathways for detoxification of reactive oxygen species and transmembrane photosensitizer efflux systems could cause resistance to aPDT. Resistance mechanisms can be evaluated by measurement of mRNA from by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Thus, the aim of this study was to access the mRNA level data obtained by RT-qPCR in bacterial cells submitted to photodynamic therapy. Studies performed on mRNA levels in bacteria after PDT were assessed on MEDLINE/Pubmed. The mRNA levels from genes related to various functions have been successfully evaluated in both Gram-positive and -negative bacteria after aPDT by RT-qPCR. Such an approach has improved the understanding of aPDT-induced effects, and reinforced the effectiveness of aPDT on bacteria, which can cause infections in different human tissues.
ISSN:1435-604X
0268-8921
1435-604X
DOI:10.1007/s10103-024-04179-9