Molecular epidemiology of Leptospira spp. serogroup Sejroe associated with chronic bovine leptospirosis

• Published in: Veterinary microbiology 2024-11, Vol.298, p.110238, Article 110238
• Main Authors: Borges, Ana Luiza dos Santos Baptista, Aymée, Luiza, Carvalho-Costa, Filipe Anibal, Lilenbaum, Walter, Di Azevedo, Maria Isabel Nogueira
• Format: Article
• Language: English
• Subjects: Animal reproduction; Animals; Bacterial Outer Membrane Proteins - genetics; Cattle; Cattle Diseases - epidemiology; Cattle Diseases - microbiology; Chronic Disease; Female; Genetic Variation; Genital leptospirosis; Genotype; Haplotypes; Leptospira - classification; Leptospira - genetics; Leptospira - isolation & purification; Leptospira interrogans - classification; Leptospira interrogans - genetics; Leptospira interrogans - isolation & purification; Leptospirosis - epidemiology; Leptospirosis - microbiology; Leptospirosis - veterinary; Molecular Epidemiology; Phylogeny; secY; Serogroup; Zoonosis
• ISSN: 0378-1135; 1873-2542; 1873-2542
• DOI: 10.1016/j.vetmic.2024.110238

Pathogenic bacteria Leptospira spp. are commonly associated with bovine leptospirosis, characterized chiefly by chronic and subclinical reproductive disorders. Strains from the Sejroe serogroup play a significant role in these chronic genital infections known as Bovine Genital Leptospirosis (BGL), which notably impact cattle health. This study aims to deepen our understanding of BGL by investigating the genetic diversity, geographical distribution, and specific anatomical sites of infection of the causative agents. Initially, uterine fragments and cervicovaginal mucus were collected from 47 cows and subjected to PCR targeting the lipL32 gene. Positive samples in lipL32-PCR (9 samples) underwent genotyping based on the secY gene. Subsequently, sequences were aligned with GenBank entries (108 sequences) and analyzed in silico. All nine sequences from this study were identified as L. interrogans with an identity >99 % to serogroup Sejroe reference strains (Norma and L53). In the broader analysis, the most prevalent species observed was L. borgpetersenii, followed by L. interrogans and L. santarosai. The haplotype network of L. interrogans revealed that haplogroups B and C exclusively included L. interrogans strains of genital origin, while haplogroup A encompassed strains from renal sources as well. These findings underscore the significance of the L. borgpetersenii genotype Hardjobovis and L. interrogans genotype Hardjoprajitno as the predominant circulating strains and highlight the existence of distinct haplogroups of pathogenic leptospires originating from genital sources. We advocate for the use of secY as an effective genetic marker for Leptospira spp. and stress the necessity for additional research prioritizing the genital tract. The outcomes of this study contribute to the development of improved control measures for chronic cattle diseases and provide valuable guidance for future investigations. •L. borgpetersenii is the most circulating species associated to chronic disease.•Genotypes Hardjobovis and Hardjoprajitno are the most frequent•L. interrogans presents haplogroups exclusively formed by genital strains.•Molecular tools based on genital samples are fundamental for chronic disease diagnosis.•secY should be encouraged as genetic marker for Leptospira phylogenetic analysis.