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Subcutaneous immunotherapy for bee venom allergy induces epitope spreading and immunophenotypic changes in allergen-specific memory B cells
[Display omitted] Allergen immunotherapy (AIT) is the only disease-modifying treatment for allergic disorders. We have recently discovered that allergen-specific memory B cells (Bmem) are phenotypically altered after 4 months of sublingual AIT for ryegrass pollen allergy. Whether these effects are s...
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| Published in: | Journal of allergy and clinical immunology 2024-12, Vol.154 (6), p.1511-1522 |
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| container_end_page | 1522 |
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| container_title | Journal of allergy and clinical immunology |
| container_volume | 154 |
| creator | McKenzie, Craig I. Reinwald, Simone Averso, Brett Spurrier, Brett Satz, Andrew von Borstel, Anouk Masinovic, Sabina Varese, Nirupama Aui, Pei Mun Wines, Bruce D. Hogarth, P. Mark Hew, Mark Rolland, Jennifer M. O’Hehir, Robyn E. van Zelm, Menno C. |
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Allergen immunotherapy (AIT) is the only disease-modifying treatment for allergic disorders. We have recently discovered that allergen-specific memory B cells (Bmem) are phenotypically altered after 4 months of sublingual AIT for ryegrass pollen allergy. Whether these effects are shared with subcutaneous allergen immunotherapy (SCIT) and affect the epitope specificity of Bmem remain unknown.
The study aimed to evaluate the phenotype and antigen receptor sequences of Bmem specific to the major bee venom (BV) allergen Api m 1 before and after ultra-rush SCIT for BV allergy.
Recombinant Api m 1 protein tetramers were generated to evaluate basophil activation in a cohort of individuals with BV allergy before and after BV SCIT. Comprehensive flow cytometry was performed to evaluate and purify Api m 1–specific Bmem. Immunoglobulin genes from single Api m 1–specific Bmem were sequenced and structurally modeled onto Api m 1.
SCIT promoted class switching of Api m 1–specific Bmem to IgG2 and IgG4 with increased expression of CD23 and CD29. Furthermore, modeling of Api m 1–specific immunoglobulin from Bmem identified a suite of possible new and diverse allergen epitopes on Api m 1 and highlighted epitopes that may preferentially be bound by immunoglobulin after SCIT.
AIT induces shifting of epitope specificity and phenotypic changes in allergen-specific Bmem. |
| doi_str_mv | 10.1016/j.jaci.2024.08.019 |
| format | article |
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Allergen immunotherapy (AIT) is the only disease-modifying treatment for allergic disorders. We have recently discovered that allergen-specific memory B cells (Bmem) are phenotypically altered after 4 months of sublingual AIT for ryegrass pollen allergy. Whether these effects are shared with subcutaneous allergen immunotherapy (SCIT) and affect the epitope specificity of Bmem remain unknown.
The study aimed to evaluate the phenotype and antigen receptor sequences of Bmem specific to the major bee venom (BV) allergen Api m 1 before and after ultra-rush SCIT for BV allergy.
Recombinant Api m 1 protein tetramers were generated to evaluate basophil activation in a cohort of individuals with BV allergy before and after BV SCIT. Comprehensive flow cytometry was performed to evaluate and purify Api m 1–specific Bmem. Immunoglobulin genes from single Api m 1–specific Bmem were sequenced and structurally modeled onto Api m 1.
SCIT promoted class switching of Api m 1–specific Bmem to IgG2 and IgG4 with increased expression of CD23 and CD29. Furthermore, modeling of Api m 1–specific immunoglobulin from Bmem identified a suite of possible new and diverse allergen epitopes on Api m 1 and highlighted epitopes that may preferentially be bound by immunoglobulin after SCIT.
AIT induces shifting of epitope specificity and phenotypic changes in allergen-specific Bmem.</description><identifier>ISSN: 0091-6749</identifier><identifier>ISSN: 1097-6825</identifier><identifier>EISSN: 1097-6825</identifier><identifier>DOI: 10.1016/j.jaci.2024.08.019</identifier><identifier>PMID: 39218358</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; allergen epitopes ; allergen-specific ; Allergens - immunology ; Animals ; Bee Venoms - immunology ; Desensitization, Immunologic - methods ; Epitopes - immunology ; Epitopes, B-Lymphocyte - immunology ; Female ; Humans ; Hypersensitivity - immunology ; Hypersensitivity - therapy ; Immunophenotyping ; Immunotherapy ; Injections, Subcutaneous ; Insect Proteins - immunology ; Male ; memory B cells ; Memory B Cells - immunology ; Middle Aged ; Phospholipases A ; Venom Hypersensitivity</subject><ispartof>Journal of allergy and clinical immunology, 2024-12, Vol.154 (6), p.1511-1522</ispartof><rights>2024 The Authors</rights><rights>Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1969-5e3d6cbac1feacef108636eff31b5ff2e9a981003e3e0a2f8482c5f45f2e60b3</cites><orcidid>0000-0003-4161-1919</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39218358$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McKenzie, Craig I.</creatorcontrib><creatorcontrib>Reinwald, Simone</creatorcontrib><creatorcontrib>Averso, Brett</creatorcontrib><creatorcontrib>Spurrier, Brett</creatorcontrib><creatorcontrib>Satz, Andrew</creatorcontrib><creatorcontrib>von Borstel, Anouk</creatorcontrib><creatorcontrib>Masinovic, Sabina</creatorcontrib><creatorcontrib>Varese, Nirupama</creatorcontrib><creatorcontrib>Aui, Pei Mun</creatorcontrib><creatorcontrib>Wines, Bruce D.</creatorcontrib><creatorcontrib>Hogarth, P. Mark</creatorcontrib><creatorcontrib>Hew, Mark</creatorcontrib><creatorcontrib>Rolland, Jennifer M.</creatorcontrib><creatorcontrib>O’Hehir, Robyn E.</creatorcontrib><creatorcontrib>van Zelm, Menno C.</creatorcontrib><title>Subcutaneous immunotherapy for bee venom allergy induces epitope spreading and immunophenotypic changes in allergen-specific memory B cells</title><title>Journal of allergy and clinical immunology</title><addtitle>J Allergy Clin Immunol</addtitle><description>[Display omitted]
Allergen immunotherapy (AIT) is the only disease-modifying treatment for allergic disorders. We have recently discovered that allergen-specific memory B cells (Bmem) are phenotypically altered after 4 months of sublingual AIT for ryegrass pollen allergy. Whether these effects are shared with subcutaneous allergen immunotherapy (SCIT) and affect the epitope specificity of Bmem remain unknown.
The study aimed to evaluate the phenotype and antigen receptor sequences of Bmem specific to the major bee venom (BV) allergen Api m 1 before and after ultra-rush SCIT for BV allergy.
Recombinant Api m 1 protein tetramers were generated to evaluate basophil activation in a cohort of individuals with BV allergy before and after BV SCIT. Comprehensive flow cytometry was performed to evaluate and purify Api m 1–specific Bmem. Immunoglobulin genes from single Api m 1–specific Bmem were sequenced and structurally modeled onto Api m 1.
SCIT promoted class switching of Api m 1–specific Bmem to IgG2 and IgG4 with increased expression of CD23 and CD29. Furthermore, modeling of Api m 1–specific immunoglobulin from Bmem identified a suite of possible new and diverse allergen epitopes on Api m 1 and highlighted epitopes that may preferentially be bound by immunoglobulin after SCIT.
AIT induces shifting of epitope specificity and phenotypic changes in allergen-specific Bmem.</description><subject>Adult</subject><subject>allergen epitopes</subject><subject>allergen-specific</subject><subject>Allergens - immunology</subject><subject>Animals</subject><subject>Bee Venoms - immunology</subject><subject>Desensitization, Immunologic - methods</subject><subject>Epitopes - immunology</subject><subject>Epitopes, B-Lymphocyte - immunology</subject><subject>Female</subject><subject>Humans</subject><subject>Hypersensitivity - immunology</subject><subject>Hypersensitivity - therapy</subject><subject>Immunophenotyping</subject><subject>Immunotherapy</subject><subject>Injections, Subcutaneous</subject><subject>Insect Proteins - immunology</subject><subject>Male</subject><subject>memory B cells</subject><subject>Memory B Cells - immunology</subject><subject>Middle Aged</subject><subject>Phospholipases A</subject><subject>Venom Hypersensitivity</subject><issn>0091-6749</issn><issn>1097-6825</issn><issn>1097-6825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kc1u3CAURlHVqpn8vEAXFctu7F7MmIDUTRs1aaVIXTR7hPFlhpGNKdiR_Ax56TKaSZddIXTPdwT3I-QDg5oBE58P9cFYXzfQbGuQNTD1hmwYqNtKyKZ9SzYAilXidqsuyGXOByh3LtV7csFVwyRv5Ya8_F46u8wm4LRk6sdxCdO8x2TiSt2UaIdInzFMIzXDgGm3Uh_6xWKmGP08RaQ5JjS9DztqQn82xH2JzGv0ltq9CbuC-3A2YKhyROtdGY44Tmml36jFYcjX5J0zQ8ab83lFnu6_P939qB5_Pfy8-_pYWaaEqlrkvbCdscyhsegYSMEFOsdZ1zrXoDJKMgCOHME0Tm5lY1u3bctIQMevyKeTNqbpz4J51qPPxweclqA5KCVbAVIWtDmhNk05J3Q6Jj-atGoG-tiBPuhjB_rYgQapSwcl9PHsX7oR-3-R16UX4MsJwPLJZ49JZ-sxWOx9QjvrfvL_8_8FQ9mckQ</recordid><startdate>202412</startdate><enddate>202412</enddate><creator>McKenzie, Craig I.</creator><creator>Reinwald, Simone</creator><creator>Averso, Brett</creator><creator>Spurrier, Brett</creator><creator>Satz, Andrew</creator><creator>von Borstel, Anouk</creator><creator>Masinovic, Sabina</creator><creator>Varese, Nirupama</creator><creator>Aui, Pei Mun</creator><creator>Wines, Bruce D.</creator><creator>Hogarth, P. Mark</creator><creator>Hew, Mark</creator><creator>Rolland, Jennifer M.</creator><creator>O’Hehir, Robyn E.</creator><creator>van Zelm, Menno C.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4161-1919</orcidid></search><sort><creationdate>202412</creationdate><title>Subcutaneous immunotherapy for bee venom allergy induces epitope spreading and immunophenotypic changes in allergen-specific memory B cells</title><author>McKenzie, Craig I. ; Reinwald, Simone ; Averso, Brett ; Spurrier, Brett ; Satz, Andrew ; von Borstel, Anouk ; Masinovic, Sabina ; Varese, Nirupama ; Aui, Pei Mun ; Wines, Bruce D. ; Hogarth, P. 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Mark</creatorcontrib><creatorcontrib>Hew, Mark</creatorcontrib><creatorcontrib>Rolland, Jennifer M.</creatorcontrib><creatorcontrib>O’Hehir, Robyn E.</creatorcontrib><creatorcontrib>van Zelm, Menno C.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of allergy and clinical immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McKenzie, Craig I.</au><au>Reinwald, Simone</au><au>Averso, Brett</au><au>Spurrier, Brett</au><au>Satz, Andrew</au><au>von Borstel, Anouk</au><au>Masinovic, Sabina</au><au>Varese, Nirupama</au><au>Aui, Pei Mun</au><au>Wines, Bruce D.</au><au>Hogarth, P. Mark</au><au>Hew, Mark</au><au>Rolland, Jennifer M.</au><au>O’Hehir, Robyn E.</au><au>van Zelm, Menno C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Subcutaneous immunotherapy for bee venom allergy induces epitope spreading and immunophenotypic changes in allergen-specific memory B cells</atitle><jtitle>Journal of allergy and clinical immunology</jtitle><addtitle>J Allergy Clin Immunol</addtitle><date>2024-12</date><risdate>2024</risdate><volume>154</volume><issue>6</issue><spage>1511</spage><epage>1522</epage><pages>1511-1522</pages><issn>0091-6749</issn><issn>1097-6825</issn><eissn>1097-6825</eissn><abstract>[Display omitted]
Allergen immunotherapy (AIT) is the only disease-modifying treatment for allergic disorders. We have recently discovered that allergen-specific memory B cells (Bmem) are phenotypically altered after 4 months of sublingual AIT for ryegrass pollen allergy. Whether these effects are shared with subcutaneous allergen immunotherapy (SCIT) and affect the epitope specificity of Bmem remain unknown.
The study aimed to evaluate the phenotype and antigen receptor sequences of Bmem specific to the major bee venom (BV) allergen Api m 1 before and after ultra-rush SCIT for BV allergy.
Recombinant Api m 1 protein tetramers were generated to evaluate basophil activation in a cohort of individuals with BV allergy before and after BV SCIT. Comprehensive flow cytometry was performed to evaluate and purify Api m 1–specific Bmem. Immunoglobulin genes from single Api m 1–specific Bmem were sequenced and structurally modeled onto Api m 1.
SCIT promoted class switching of Api m 1–specific Bmem to IgG2 and IgG4 with increased expression of CD23 and CD29. Furthermore, modeling of Api m 1–specific immunoglobulin from Bmem identified a suite of possible new and diverse allergen epitopes on Api m 1 and highlighted epitopes that may preferentially be bound by immunoglobulin after SCIT.
AIT induces shifting of epitope specificity and phenotypic changes in allergen-specific Bmem.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>39218358</pmid><doi>10.1016/j.jaci.2024.08.019</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-4161-1919</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adult allergen epitopes allergen-specific Allergens - immunology Animals Bee Venoms - immunology Desensitization, Immunologic - methods Epitopes - immunology Epitopes, B-Lymphocyte - immunology Female Humans Hypersensitivity - immunology Hypersensitivity - therapy Immunophenotyping Immunotherapy Injections, Subcutaneous Insect Proteins - immunology Male memory B cells Memory B Cells - immunology Middle Aged Phospholipases A Venom Hypersensitivity |
| title | Subcutaneous immunotherapy for bee venom allergy induces epitope spreading and immunophenotypic changes in allergen-specific memory B cells |
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