The mechanism of cell-damaging reactive oxygen generation by colloidal fullerenes

Abstract Because of the ability to induce cell death in certain conditions, the fullerenes (C60 ) are potential anticancer and toxic agents. The colloidal suspension of crystalline C60 (nano-C60 , n C60 ) is extremely toxic, but the mechanisms of its cytotoxicity are not completely understood. By co...

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Published in:Biomaterials 2007-12, Vol.28 (36), p.5437-5448
Main Authors: Markovic, Zoran, Todorovic-Markovic, Biljana, Kleut, Duska, Nikolic, Nadezda, Vranjes-Djuric, Sanja, Misirkic, Maja, Vucicevic, Ljubica, Janjetovic, Kristina, Isakovic, Aleksandra, Harhaji, Ljubica, Babic-Stojic, Branka, Dramicanin, Miroslav, Trajkovic, Vladimir
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Carbon
Cell Line
Cell Survival - drug effects
Colloids
Cytotoxicity
Dentistry
Free radical
Fullerenes - toxicity
Humans
Mice
Modelling
Nanoparticle
Reactive Oxygen Species - metabolism
Solvents
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Summary:Abstract Because of the ability to induce cell death in certain conditions, the fullerenes (C60 ) are potential anticancer and toxic agents. The colloidal suspension of crystalline C60 (nano-C60 , n C60 ) is extremely toxic, but the mechanisms of its cytotoxicity are not completely understood. By combining experimental analysis and mathematical modelling, we investigate the requirements for the reactive oxygen species (ROS)-mediated cytotoxicity of different n C60 suspensions, prepared by solvent exchange method in tetrahydrofuran (THF/ n C60 ) and ethanol (EtOH/ n C60 ), or by extended mixing in water (aqu/ n C60 ). With regard to their capacity to generate ROS and cause mitochondrial depolarization followed by necrotic cell death, the n C60 suspensions are ranked in the following order: THF/ n C60 >EtOH/ n C60 >aqu/ n C60 . Mathematical modelling of singlet oxygen (1 O2 ) generation indicates that the1 O2 -quenching power (THF/ n C60
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2007.09.002